伯森電子報 2017年2月號第4期                             無法看到畫面顯示?請點選這裡開啟網頁   
伯森2017細胞轉染試劑春季限定優惠:訂購 TransIT-X2® 轉染試劑 (1.5ml ) 即可享 ibidi 指定商品半價加購優惠*,加購品項適用於傷口癒合、免疫螢光染色、活細胞影像觀察等實驗,優惠詳情請洽伯森業務專員  ※加購品項請見本刊說明,限量加購壹組。
【TransIT-X2® 新型 DNA/RNA 雙效轉染試劑】TransIT-X2® 是由美商 Mirus Bio 公司獨家研發的新型高效率轉染試劑,可同時適用於 plasmid DNA 與 siRNA/miRNA 的細胞轉染實驗。產品特性包含:■採用創新非脂質體聚合物配方 (non-liposomal polymer),轉染效率更佳,並能大幅降低細胞毒殺性 ■對於 plasmid DNA 及 siRNA/miRNA 皆具有高轉染效率,可以進行 Co-trans-fection 實驗 ■不含任何動物來源成份,可安心使用於 "animal origin-free" 實驗。[Figure: TransIT-X2® achieves better/comparable DNA delivery to Lipofectamine® 2000 in 36/41 cell types tested]
【Figure】Functional Co-delivery of Plasmid DNA and siRNA Using the TransIT-X2® Dynamic Delivery System - TransIT-X2® Dynamic Delivery System was used to transfect plasmid Cy®5 labeled DNA encoding nuclear YFP and Cy®3 labeled siRNA into HeLa cells. Image key: yellow (nuclear YFP), blue (Cy®5 labeled DNA), red (Cy®3 labeled siRNA), green (actin cytoskeleton).  【Figure】Efficient Genome Editing with Cas9 Plasmid DNA + Guide RNA Oligonucleotides - HEK293T/17, U2OS and NHDF cells were co-transfected with 0.5 µg of Cas9 encoding pDNA and 50nM PPIB targeting 2-part gRNA using TransIT-X2® Dynamic Delivery System (2 µl/well of a 24-well plate, Mirus Bio).  A T7E1 mismatch detection assay was used to measure cleavage efficiency at 48 hours post-transfection.
【Figure】TransIT-X2® Dynamic Delivery System Achieves Higher Knockdown than Lipofectamine® 2000 - TransIT-X2® Dynamic Delivery System and Lipofectamine® 2000 Transfection Reagent were used to transfect siRNA targeting endogenous proteins – GAPDH and AHA1 or to deliver a non-targeting siRNA control in normal human dermal fibroblasts (NHDF). 【Figure】Effective miRNA Delivery Using TransIT-X2® Dynamic Delivery System Yields Decreased Levels of PTK9 mRNA - TransIT-X2® Dynamic Delivery System and Lipofectamine® 2000 Transfection Reagent were used to transfect Pre-miR™ hsa-miR-1 miRNA Precursor or mirVana™ miRNA mimic, miR-1, both known to decrease PTK9 mRNA levels. A Pre-miR™ negative control was also transfected to assess baseline mRNA levels.
【Culture-Insert 2 Well | 3 Well | 4 Well】■可節省細胞 (2.1~4.9 x 10^4 cells/well) 與培養基 (70~110 µl/well) 的使用量 ■細胞間隔框架以矽膠組成,並採無膠接合設計,無需擔心物質殘留造成細胞毒害 ■可自行貼附在任何光滑的乾燥表面,使用彈性便利 ■相較 Scratch assay,不會有刮傷細胞或 coating 表面的疑慮 ■操作簡單,可精確量化實驗結果,有效提高實驗再現性 ■應用實驗:Wound Healing Assays, Migration Assays, 2D Invasion Assays, Co-Cultivation of Cells。
訂購資訊 - Culture-Insert 2 Well | 3 Well | 4 Well
【Immunofluorescence Chamber】■由矽膠材質透明框架與玻片所組成,撕下框架輕鬆不費力 ■可同時進行 3/ 8/ 12 組平行測試實驗 ■3 well chamber 另提供專用玻片,可在節省抗體染劑用量的同時 (150 µl/well),仍保有大範圍觀察面積 ■適合細胞培養與免疫螢光染色實驗使用,染色結果可以一般蓋玻片封片後長期保存 ■適用於正立式及倒立式顯微鏡觀察。
【µ-Slide and µ-Dish】■超薄底設計,厚度符合 No.1.5 標準蓋玻片厚度,上高倍率顯微鏡觀察也沒問題 ■ibiTreat 底部適合各類細胞貼附及生長 ■極佳的物理及化學耐受性,相容於多種常見之固定試劑 (methanol, acetone, paraformaldehyde)。
伯森生物科技股份有限公司聯絡資訊
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