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F-actin 螢光染色實驗工具 | 固定細胞染色、活細胞影像攝影

F-actin 螢光染色實驗工具特輯

G-actin has the ability to polymerize, resulting in the F-actin polymer. IMAGE © ibidi GmbH.在絕大部分的真核細胞中,肌動蛋白 (Actin) 皆是含量最為豐富的一種蛋白質,在生物演化過程中被高度保留下來。根據其結構,肌動蛋白可區分為單體球狀肌動蛋白 (globular actin, G-actin)、以及由球狀肌動蛋白聚合而成的纖維形肌動蛋白 (filamentous actin, F-actin) 兩種型式。其中 F-actin 在細胞骨架 (cytoskeleton) 中扮演著極為重要的角色,了解 F-actin 在細胞內的表現量變化與分布,將有助於我們對細胞遷移、細胞形態、細胞附著、癌症治療等多方面研究的了解。

針對固定細胞染色以及活細胞影像攝影兩種常見的 F-actin 螢光染色實驗,伯森提供完整的實驗工具選擇,幫助您更高效便捷地取得優質細胞影像。

固定細胞染色:CytoPainter Phalloidin

Phalloidin 為一種帶有毒性的胜肽,具有與 F-actin 專一性結合的特性,「CytoPainter Phalloidin」為標定有螢光染料的 Phalloidin 試劑產品,能夠協助研究人員精確掌握細胞內 F-actin 的分布情形、了解 F-actin 表現量變化。

  • 適用於多種樣本類型 — Fixed and permeabilized tissue sectionsAdherent/Suspension cells 等。
  • 螢光標定選擇多樣化 — FITCRhodamineiFluor 405iFluor 488iFluor 555iFluor 647 等。
  • 兩種產品包裝型式 — [Kit] 內含 10 倍濃縮螢光染劑及稀釋液; [Reagent] 僅含 10 倍濃縮螢光染劑。

CytoPainter F-actin Staining Kit - Blue Fluorescence (ab112124)

CytoPainter F-actin Staining Kit - Blue Fluorescence (ab112124). F-actin was stained with CytoPainter blue in mouse embryonic stem cell-differentiated embryoid bodies using ICC/IF. Image courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School.

CytoPainter Phalloidin-iFluor 488 Reagent (ab176753)

CytoPainter Phalloidin-iFluor 488 Reagent (ab176753). Actin filaments staining in HeLa cells. Actin filaments (green) were stained with CytoPainter Phalloidin-iFluor 488 reagent (ab176753); tubulin filaments were stained with a mouse anti-tubulin antibody/goat anti-mouse IgG (red). Nuclei were stained with Hoechst 33342.

CytoPainter Phalloidin-iFluor 647 Reagent (ab176759)

CytoPainter Phalloidin-iFluor 647 Reagent (ab176759). Actin filaments staining in HeLa cells. Actin filaments (red) were stained with CytoPainter Phalloidin-iFluor 647 reagent (ab176759). Nuclei were stained with DAPI (blue).

  Product Name Ex/Em Pack Size Cat. No.
F-actin Staining Kit - Blue Fluorescence - Cytopainter 350/450 500 tests ab112124
F-actin Staining Kit - Green Fluorescence - Cytopainter 490/525 500 tests ab112125
F-actin Staining Kit - Orange Fluorescence - Cytopainter 550/575 500 tests ab112126
F-actin Staining Kit - Red Fluorescence - Cytopainter 594/610 500 tests ab112127
Phalloidin-iFluor 350 Reagent 353/442 300 tests ab176751
Phalloidin-iFluor 405 Reagent 400/421 300 tests ab176752
Phalloidin FITC Reagent 492/518 300 tests ab235137
Phalloidin-iFluor 488 Reagent 493/517 300 tests ab176753
Phalloidin-iFluor 514 Reagent 520/547 300 tests ab176754
Phalloidin-iFluor 532 Reagent 542/558 300 tests ab176755
Rhodamine Phalloidin Reagent 546/575 300 tests ab235138
Phalloidin-iFluor 555 Reagent 556/574 300 tests ab176756
Phalloidin-iFluor 594 Reagent 590/618 300 tests ab176757
Phalloidin-iFluor 633 Reagent 634/649 300 tests ab176758
Phalloidin-iFluor 647 Reagent 650/665 300 tests ab176759
Phalloidin-iFluor 680 Reagent 683/700 300 tests ab176760
Phalloidin-iFluor 700 Reagent 692/708 300 tests ab176761
Phalloidin-iFluor 750 Reagent 752/778 300 tests ab176762
Phalloidin-iFluor 790 Reagent 787/808 300 tests ab176763

活細胞影像攝影:LifeAct

LifeAct specifically stains F-actin.LifeAct 為長度僅有 17 個胺基酸的無毒胜肽,可專一性地與 F-actin 結合。藉由在細胞內表達標定有綠色螢光 (TagGFP2) 或紅色螢光 (TagRFP) 的 LifeAct,即可在顯微鏡下清晰地觀察到活細胞內 F-actin 的即時分布與表現量變化。

  • 低干擾 — 相較其它顯色方法,對 F-actin 原有的組織架構及動態行為干擾性最低。
  • 無毒性 — 不會影響細胞本身的正常生理功能。
  • 款式多元 — 多種產品型式,便利科研人員根據細胞特性選擇最佳方案。
     
LifeAct Plasmids

 

LifeAct Plasmids 為現成的 Plasmid,到貨後可馬上轉染細胞進行實驗!兩種 Promoters 選擇:(1). CMV - 可驅使基因大量表現; (2). CAG - 適合對外來基因大量表現較為敏感的細胞類型,例如淋巴球細胞或神經細胞。
     
LifeAct Viral Vectors

 

LifeAct Viral Vectors (1). LifeAct Adenoviral Vectors - 適合難轉染的細胞使用,例如初代細胞或幹細胞; (2). LifeAct Lentiviral Vectors - 適合用來建立需要長期穩定表現 LifeAct 的細胞株。
     
LifeAct Stable HT-1080 Cell Line

 

LifeAct Stable HT-1080 Cell Line 為使用人類纖維肉瘤 HT1080 細胞建立的 LifeAct 穩定表現細胞株,可直接適用於各類影像或藥物分析實驗。

Restructuring of the Human Macrophage Cytoskeleton During Borreliae Uptake

Restructuring of the Human Macrophage Cytoskeleton During Borreliae Uptake. Phagocytosis of borreliae by a primary human macrophage. Time-lapse movie of a confocal z-stack showing a primary human macrophage expressing RFP-LifeAct (red) internalizing several GFP-expressing spirochetes (green) with actin-rich cell protrusions. Sequence 41 min. Data by Dr. Mirko Himmel and Prof. Stefan Linder, PhD, Universitätsklinikum Hamburg-Eppendorf, Germany.

Live Cell Imaging of Actin Dynamics in a Chemotactic Gradient

Live Cell Imaging of Actin Dynamics in a Chemotactic Gradient. Live cell imaging of actin dynamics in a LifeAct-expressing primary dendritic mouse cell after the application of a chemotactic gradient.

F-Actin Visualization in a 3D Hydrogel Matrix

F-Actin Visualization in a 3D Hydrogel Matrix. Z-stack of HT-1080 LifeAct-TagGFP2 cells in a 3D hydrogel environment.

Product Name Pack Size Cat. No.
pCMV-LifeAct-TagGFP2: plasmid
20 µg
IB-60101
pCMV-LifeAct-TagRFP: plasmid 20 µg IB-60102
pCAG-LifeAct-TagGFP2: plasmid 20 µg IB-60106
pCAG-LifeAct-TagRFP: plasmid 20 µg IB-60107
rAVCMV-LifeAct-TagGFP2: adenoviral vector 1 x 10⁹ IU IB-60121
rAVCMV-LifeAct-TagRFP: adenoviral vector 1 x 10⁹ IU IB-60122
rLVUbi-LifeAct-TagGFP2: lentiviral vector 1 x 10⁶ TU IB-60141
rLVUbi-LifeAct-TagRFP: lentiviral vector 1 x 10⁶ TU IB-60142
HT-1080 LifeAct-TagGFP2: HT-1080 cells expressing LifeAct-TagGFP2 5 x 10⁵ cells IB-40101

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