AlphaLISA 免疫分析試劑

AlphaLISA® 是由美商 Revvity 公司所研發出的新型態 ELISA 技術，此技術與 ELISA 相同可以用來偵測細胞培養液、血清、血漿等生物樣本中的特定分子，但卻擁有更簡單的操作步驟以及更加優異的偵測靈敏度、與更廣泛的偵測區間，克服了現行 ELISA 技術樣本需求量大 (50~200 µl)、偵測範圍狹窄必須重複測試樣本稀釋比例、以及步驟繁複需多次 Wash 步驟等缺點。

產品特點：

• 偵測範圍更廣，不必擔心樣本稀釋比例不夠，超出分析範圍。
  
• 靈敏度更高，樣本更省 (5 µl in 96-well format)。
• 使用更彈性，可依據樣本數目多寡彈性選擇以 96-1536 微量多孔盤進行實驗。
  
• 步驟更簡單，不需傳統 ELISA 的麻煩洗滌步驟。
  
• 實驗流程可完全自動化，方便大量樣本篩選時使用。
  
• 產品種類豐富，涵蓋各類研究領域熱門標的分子。
• 經 Merck, Eli Lilly 等國際知名藥廠同步採用，品質值得信賴！

AlphaLISA ELISA Sensitivity  Highly sensitive (sub-pM)  Sensitive (pM range)  Selectivity  High  High  Assay Nature  Homogeneous: no washing  Heterogeneous: multiple washings  Labor  Limited labor requirement  Very labor-intensive  High Throughput  Easy to miniaturize  Difficult to employ  Antibody Requirement  Can be used with high- and low-affinity antibodies  Requires high-affinity antibodies  Sample Volume  Small, >5 μL  Large, 50-200 μL  Analytical Range  Large (4 orders of magnitude)  Limited (2 orders of magnitude)  Instrumentation  EnSpire™ Alpha or EnSpire™ Alpha Alpha PLUS plate reader  Standard microplate reader
AlphaLISA 具有比 ELISA 更優異的偵測靈敏度、與更廣泛的偵測範圍！		AlphaLISA 具有比 ELISA 更簡單快速的實驗流程，全程不需 wash 步驟且僅需 5 µl 樣本即可進行偵測。

作用原理：

AlphaLISA® 作用原理類似 Sandwich ELISA，是藉由 Antibody-Pair 與目標分子間的專一性結合來完成目標分子的定量偵測。當樣本中含有目標分子存在時， 兩個分別鍵結有專一性抗體的 Alpha 技術核心物質 "Alpha Donor Bead" 與 "Alpha Acceptor Bead" 即會被拉近，此時於 680 nm 雷射光激發下，Donor Bead 表面所帶有的光敏感物質即會催化周遭的氧分子形成活化態，並進一步激發專一性結合的 Acceptor Bead 產生化學冷光反應，此能量會轉移至 Acceptor Bead 所帶有的螢光物質，使其最終產生 615nm 的螢光訊號。

產品效能：

AlphaLISA 具有極佳的偵測靈敏度！AlphaLISA assays can be performed with 5 µl sample volume (regular sample volume) or higher sample (e.g. 10 µl or 20 µl) to increase sensitivity. *LDL = Lower Detection Limit.

AlphaLISA 具有良好的專一性！No cross-reactivity observed up to 100 ng/ml of rat or murine IL17A, or other human IL17 isoforms. *LDL = Lower Detection Limit.

訂購資訊 (請點擊下列分類選項，瀏覽詳細訂購資訊)

分類 產品品項 Angiogenesis EPO TNFα VEGF VEGFA VEGFB VEGFC VEGFD

Product Pack Size Cat. No. AlphaLISA Human EPO Research Immunoassay Kit 500 assay PK-AL206C 5,000 assay PK-AL206F AlphaLISA Human TNF α Research Immunoassay Kit 500 assay PK-AL208C 5,000 assay PK-AL208F AlphaLISA TNF α (mouse) Research Immunoassay Kit 500 assay PK-AL505C 5,000 assay PK-AL505F AlphaLISA Human VEGF Research Immunoassay Kit 500 assay PK-AL201C 5,000 assay PK-AL201F AlphaLISA VEGFA (mouse) Research Immunoassay Kit 500 assay PK-AL520C 5,000 assay PK-AL520F AlphaLISA Human VEGFB Research Immunoassay Kit 500 assay PK-AL251C 5,000 assay PK-AL251F AlphaLISA Human VEGFC Research Immunoassay Kit 500 assay PK-AL252C 5,000 assay PK-AL252F AlphaLISA Human VEGFD Research Immunoassay Kit 500 assay PK-AL253C 5,000 assay PK-AL253F

Biologics / Bioprocess Albumin CHO-P E. coli HCP IgA IgE IgG IgM NSO-P Residual Protein A

Product Pack Size Cat. No. AlphaLISA Human Albumin Kit 500 assay PK-AL294C 5,000 assay PK-AL294F AlphaLISA CHO-P Immunoassay Research Kit 500 assay PK-AL210C 5,000 assay PK-AL210F AlphaLISA Human E coli protiens (Host Cell Proteins) Research Kit 500 assay PK-AL261C 5,000 assay PK-AL261F AlphaLISA Human IgA Research Kit 500 assay PK-AL262C 5,000 assay PK-AL262F AlphaLISA Human IgE Kit 500 assay PK-AL292C 5,000 assay PK-AL292F AlphaLISA Human IgG Research Immunoassay Kit 500 assay PK-AL205C 5,000 assay PK-AL205F AlphaLISA Human IgM Research Kit 500 assay PK-AL263C 5,000 assay PK-AL263F AlphaLISA NS0-P Immunoassay Research Kit 500 assay PK-AL226C 5,000 assay PK-AL226F AlphaLISA Residual Protein A Immunoassay Research Kit 500 assay PK-AL287C 5,000 assay PK-AL287F

Cancer AFP CA125 Caspase-3 active CXCL11/I-TAC EPO ERBB2/HER2 HGFR/c-MET IFN-β MMP1 MMP2 MMP3 MMP9 β-NGF PSA TNFα TFF3 TIMP1

Product Pack Size Cat. No. AlphaLISA Human Alpha-fetoprotein (AFP) Immunoassay Research Kit 500 assay PK-AL230C 5,000 assay PK-AL230F AlphaLISA Human Caspase-3 active Kit 500 assay PK-AL278C 5,000 assay PK-AL278F AlphaLISA Human C-X-C motif chemokine 11 (Interferon inducible T-cell a chemoattractant) Research Kit 500 assay PK-AL260C 5,000 assay PK-AL260F AlphaLISA Human EPO Research Immunoassay Kit 500 assay PK-AL206C 5,000 assay PK-AL206F AlphaLISA Human ERBB2 / HER2 Research Immunoassay Kit 500 assay PK-AL235C 5,000 assay PK-AL235F AlphaLISA Human HGFR / c-MET Research Immunoassay Kit 500 assay PK-AL281C 5,000 assay PK-AL281F AlphaLISA Human Interferon β Research Kit 500 assay PK-AL265C 5,000 assay PK-AL265F AlphaLISA Human Metalloproteinase inhibitor 1 (Tissue inhibitor of metalloproteinases, TIMP1) Research Kit 500 assay PK-AL272C 5,000 assay PK-AL272F AlphaLISA Human MMP1 Research Immunoassay Kit 500 assay PK-AL242C 5,000 assay PK-AL242F AlphaLISA Human MMP2 Research Kit 500 assay PK-AL269C 5,000 assay PK-AL269F AlphaLISA Human MMP3  Immunoassay Research Kit 500 assay PK-AL284C 5,000 assay PK-AL284F AlphaLISA Human MMP9 Research Immunoassay Kit 500 assay PK-AL243C 5,000 assay PK-AL243F AlphaLISA MMP9 (mouse) Kit 500 assay PK-AL519C 5,000 assay PK-AL519F AlphaLISA Human Mucin-16 (Ovarian carcinoma antigen CA125) Research Kit 500 assay PK-AL256C 5,000 assay PK-AL256F AlphaLISA Human β-NGF Research Immunoassay Kit 500 assay PK-AL246C 5,000 assay PK-AL246F AlphaLISA Human PSA  Immunoassay Research Kit 500 assay PK-AL28C 5,000 assay PK-AL228F AlphaLISA Human TNF α Research Immunoassay Kit 500 assay PK-AL208C 5,000 assay PK-AL208F AlphaLISA TNF α (mouse) Research Immunoassay Kit 500 assay PK-AL505C 5,000 assay PK-AL505F AlphaLISA Human Trefoil factor 3 (TFF3) Research Kit 500 assay PK-AL273C 5,000 assay PK-AL273F

Cardiovascular Cardiac Troponin I D-dimer EPO ICAM-1 Myeloperoxidase Myoglobin PAI-1 PCSK9 Plasminogen Renin/Prorenin tPA

Product Pack Size Cat. No. AlphaLISA Human cardiac Troponin I Kit 500 assay PK-AL295C 5,000 assay PK-AL295F AlphaLISA Human D-dimer Kit 500 assay PK-AL290C 5,000 assay PK-AL290F AlphaLISA Human EPO Research Immunoassay Kit 500 assay PK-AL206C 5,000 assay PK-AL206F AlphaLISA Human ICAM-1 Immunoassay Research Kit 500 assay PK-AL282C 5,000 assay PK-AL282F AlphaLISA Human Myeloperoxidase Research Immunoassay Kit 500 assay PK-AL245C 5,000 assay PK-AL245F AlphaLISA Human Myoglobin Kit 500 assay PK-AL285C 5,000 assay PK-AL285F AlphaLISA Human PAI-1 Kit 500 assay PK-AL286C 5,000 assay PK-AL286F AlphaLISA Human Proprotein convertase subtilisin/kexin 9 (PCSK9) Research Kit 500 assay PK-AL270C 5,000 assay PK-AL270F AlphaLISA Human Plasminogen Research Immunoassay Kit 500 assay PK-AL248C 5,000 assay PK-AL248F AlphaLISA Human Renin Research Immunoassay Kit 500 assay PK-AL249C 5,000 assay PK-AL249F AlphaLISA Human Tissue Plasminogen Activator (tPA)  Research Immunoassay Kit 500 assay PK-AL250C 5,000 assay PK-AL250F

Central Nervous System Amyloid β 1-15/16 Amyloid β 1-40 Amyloid β 1-42 Amyloid β 1-x   sAPPα sAPPβ Tau Amyloid β oligomers

Product Pack Size Cat. No. AlphaLISA Human Amyloid β 1-15/16 Immunoassay Research Kit 500 assay PK-AL274C 5,000 assay PK-AL274F AlphaLISA Human Amyloid β 1-40 (Aβ 1-40) Research Immunoassay Kit 500 assay PK-AL202C 5,000 assay PK-AL202F AlphaLISA Human Amyloid β 1-40 (high specificity) Immunoassay Research Kit 500 assay PK-AL275C 5,000 assay PK-AL275F AlphaLISA Amyloid β 1-40 (mouse/rat) Immunoassay Research Kit 500 assay PK-AL506C 5,000 assay PK-AL506F AlphaLISA Human Amyloid β 1-42 (Aβ 1-42) Research Immunoassay Kit 500 assay PK-AL203C 5,000 assay PK-AL203F AlphaLISA Human Amyloid β 1-42 (high specificity) Immunoassay Research Kit 500 assay PK-AL276C 5,000 assay PK-AL276F AlphaLISA Amyloid β 1-42 (mouse/rat) Immunoassay Research Kit 500 assay PK-AL507C 5,000 assay PK-AL507F AlphaLISA Human Amyloid ß 1-x Kit 500 assay PK-AL288C 5,000 assay PK-AL288F AlphaLISA Human Amyloid ß Oligomers Kit 500 assay PK-AL296C 5,000 assay PK-AL296F AlphaLISA Human sAPPα (C-term specific) (Amyloid Precursor Protein α, Soluble) Research Kit 500 assay PK-AL254C 5,000 assay PK-AL254F AlphaLISA Human SAPPβ (Amyloid Precursor Protein β, Secreted) Research Kit 500 assay PK-AL232C 5,000 assay PK-AL232F AlphaLISA Human SAPPβ (high specificity) (Amyloid Precursor Protein β, Soluble) Research Kit 500 assay PK-AL255C 5,000 assay PK-AL255F AlphaLISA Human TAU Immunoassay Research Kit 500 assay PK-AL271C 5,000 assay PK-AL271F

Inflammation CXCL1/GRO-α CCL2/MCP1 CCL3/MIP-1α CCL4/MIP-1β CCL5 / RANTES CRP CXCL9/MIG CXCL10/IP-10 G-CSF GM-CSF IFN-α IFN-γ IL1α IL1β IL2 IL3 IL4 IL5 IL6 IL7 IL8 IL10 IL11 IL12 (p70) IL13 IL15 IL17 IL17A IL18 TNFα

Product Pack Size Cat. No. AlphaLISA Human CXCL1 / GRO-α Immunoassay Research Kit 500 assay PK-AL279C 5,000 assay PK-AL279F AlphaLISA Human CCL2/MCP1 (Monocyte Chemoattractant Protein-1) Research Immunoassay Kit 500 assay PK-AL244C 5,000 assay PK-AL244F AlphaLISA CCL2/MCP1 (mouse/rat) Research Immunoassay Kit 500 assay PK-AL509C 5,000 assay PK-AL509F AlphaLISA Human C-C motif chemokine 3 (Macrophage inflammatory protein 1-a) Research Kit 500 assay PK-AL257C 5,000 assay PK-AL257F AlphaLISA Human C-C motif chemokine 4 (Macrophage inflammatory protein 1-b) Research Kit 500 assay PK-AL258C 5,000 assay PK-AL258F AlphaLISA Human CCL5 / RANTES Kit 500 assay PK-AL289C 5,000 assay PK-AL289F AlphaLISA CCL5 / RANTES (mouse) Kit 500 assay PK-AL516C 5,000 assay PK-AL516F AlphaLISA Human C-Reactive Protein (CRP) Immunoassay Research Kit 500 assay PK-AL233C 5,000 assay PK-AL233F AlphaLISA Human CXCL9 / MIG Immunoassay Research Kit 500 assay PK-AL280C 5,000 assay PK-AL280F AlphaLISA Human C-X-C motif chemokine 10 (10 kDa IFN g-induced protein) Research Kit 500 assay PK-AL259C 5,000 assay PK-AL259F AlphaLISA Human G-CSF Immunoassay Research Kit 500 assay PK-AL213C 5,000 assay PK-AL213F AlphaLISA Human GM-CSF Immunoassay Research Kit 500 assay PK-AL216C 5,000 assay PK-AL216F AlphaLISA GM-CSF (mouse) Kit 500 assay PK-AL517C 5,000 assay PK-AL517F AlphaLISA Human IFN-α Research Kit 500 assay PK-AL264C 5,000 assay PK-AL264F AlphaLISA Human IFN-γ Immunoassay Research Kit 500 assay PK-AL217C 5,000 assay PK-AL217F AlphaLISA IFN-γ (mouse) Research Kit 500 assay PK-AL501C 5,000 assay PK-AL501F AlphaLISA Human IL1α Research Kit 500 assay PK-AL238C 5,000 assay PK-AL238F AlphaLISA Human IL1β Immunoassay Research Kit 500 assay PK-AL220C 5,000 assay PK-AL220F AlphaLISA IL1β (mouse) Research Kit 500 assay PK-AL503C 5,000 assay PK-AL503F AlphaLISA IL1β (rat) Kit 500 assay PK-AL518C 5,000 assay PK-AL518F AlphaLISA Human IL2 Immunoassay Research Kit 500 assay PK-AL221C 5,000 assay PK-AL221F AlphaLISA IL2 (mouse) Immunoassay Research Kit 500 assay PK-AL511C 5,000 assay PK-AL511F AlphaLISA Human IL3 Immunoassay Research Kit 500 assay PK-AL222C 5,000 assay PK-AL222F AlphaLISA Human IL4 Research Kit 500 assay PK-AL266C 5,000 assay PK-AL266F AlphaLISA Human IL5 Research Kit 500 assay PK-AL267C 5,000 assay PK-AL267F AlphaLISA Human IL6 Immunoassay Research Kit 500 assay PK-AL223C 5,000 assay PK-AL223F AlphaLISA IL6 (mouse) Research Kit 500 assay PK-AL504C 5,000 assay PK-AL504F AlphaLISA Human IL7 Research Kit 500 assay PK-AL268C 5,000 assay PK-AL268F AlphaLISA Human IL8 Immunoassay Research Kit 500 assay PK-AL224C 5,000 assay PK-AL224F AlphaLISA Human IL10 Immunoassay Research Kit 500 assay PK-AL218C 5,000 assay PK-AL218F AlphaLISA IL10 (mouse) Immunoassay Research Kit 500 assay PK-AL502C 5,000 assay PK-AL502F AlphaLISA Human IL11 Kit 500 assay PK-AL293C 5,000 assay PK-AL293F AlphaLISA Human IL12 (p70) Immunoassay Research Kit 500 assay PK-AL239C 5,000 assay PK-AL239F AlphaLISA Human IL13 Immunoassay Research Kit 500 assay PK-AL240C 5,000 assay PK-AL240F AlphaLISA Human IL15 Immunoassay Research Kit 500 assay PK-AL283C 5,000 assay PK-AL283F AlphaLISA IL15 (mouse) Immunoassay Research Kit 500 assay PK-AL513C 5,000 assay PK-AL513F AlphaLISA Human IL17 Immunoassay Research Kit 500 assay PK-AL219C 5,000 assay PK-AL219F AlphaLISA IL17A (mouse/rat) Immunoassay Research Kit 500 assay PK-AL514C 5,000 assay PK-AL514F AlphaLISA Human IL18 Immunoassay Research Kit 500 assay PK-AL241C 5,000 assay PK-AL241F AlphaLISA Human TNF α Research Immunoassay Kit 500 assay PK-AL208C 5,000 assay PK-AL208F AlphaLISA TNF α (mouse) Research Immunoassay Kit 500 assay PK-AL505C 5,000 assay PK-AL505F

Metabolic Adiponectin Albumin C-peptide GLP-1 Growth Hormone IGF1 IGF2 Insulin Leptin Prolactin

Product Pack Size Cat. No. AlphaLISA Human Adiponectin Immunoassay Research Kit 500 assay PK-AL209C 5,000 assay PK-AL209F AlphaLISA Adiponectin (mouse) Kit 500 assay PK-AL515C 5,000 assay PK-AL515F AlphaLISA Human Albumin Kit 500 assay PK-AL294C 5,000 assay PK-AL294F AlphaLISA C-Peptide (mouse/rat) Kit 500 assay PK-AL510C 5,000 assay PK-AL510F AlphaLISA Human GLP-1 (7-36 amide) Immunoassay Research Kit 500 assay PK-AL215C 5,000 assay PK-AL215F AlphaLISA Human Growth Hormone (GH) Immunoassay Research Kit 500 assay PK-AL214C 5,000 assay PK-AL214F AlphaLISA Human Insulin Research Immunoassay Kit 500 assay PK-AL204C 5,000 assay PK-AL204F AlphaLISA Human Insulin-like Growth Factor 1 Research Kit 500 assay PK-AL236C 5,000 assay PK-AL236F AlphaLISA Human Insulin-like Growth Factor 2 Research Kit 500 assay PK-AL237C 5,000 assay PK-AL237F AlphaLISA Human Leptin Immunoassay Research Kit 500 assay PK-AL225C 5,000 assay PK-AL225F AlphaLISA Human Prolactin Immunoassay Research Kit 500 assay PK-AL227C 5,000 assay PK-AL227F

Virology HIV p24

Product Pack Size Cat. No. AlphaLISA HIV p24 Research Immunoassay Kit 500 assay PK-AL207C 5,000 assay PK-AL207F AlphaLISA HIV p24 (high sensitivity) Kit 500 assay PK-AL291C 5,000 assay PK-AL291F