CNM & CNMCS Compartmental Protein
Extraction Kit

• 適合動物組織或細胞作為樣本來源，三小時內即可快速完成萃取實驗。
• CNM Kit 可分離出分別位於細胞質、細胞核、細胞膜的三類蛋白質； CNMCS Kit 則可分離出分別位於細胞質、細胞核、細胞膜、細胞骨架的的四類蛋白質。
• 所萃取得的蛋白質產物仍可保有生物活性，適用於 Immunoprecipitation (IP)、Western Blot、2D Electrophoresis 等實驗。
• 產品內附 Protease Inhibitor Cocktail，可節省額外購買的花費與時間。

Example of CNMCS Protein Extraction from Mammalian Tissue. Total Protein and compartmental proteins extracted from rat colon tissue using Biochain’s Total Protein Extraction Kit and CNMCS Compartmental Protein Extraction Kit were submitted to SDS-PAGE in 5 identical gels. Coomassie staining of one piece of gel indicated distinct protein pattern of respective fractions. Immunoblotting of PVDF membranes transferred from 5 other pieces of gel against cytoplasmic marker protein GAPDH, nuclear marker protein Histone H1, membrane marker protein Na+/K+ ATPase, and cytoskeleton marker protein vimentin assigned the majorities of the marker proteins to their expected compartmental fractions. Lane 1: Total Protein; Lane 2: Cytoplasmic Protein; Lane 3: Nuclear Protein; Lane 4: Membrane Protein; Lane 5: Cytoskeleton Protein.

訂購資訊