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友善列印CyClone Ligase-free Cloning Kit

  • 高效率重組基因構築試劑,可將任何 DNA 片段無縫接合至任一載體,無多餘序列產生,且不需使用到限制酶或 DNA 連接酶 ( ligase ) 。
  • 十倍濃縮試劑設計,可容納更多 DNA 樣本體積,操作更便利。
  • 廣泛適用於各種勝任細胞,且反應產物不需經過任何額外純化或稀釋步驟,即可直接進行 Transformation 。
  • 高 Transformation 效率,菌落數 ( colonies ) 約 100~1000 顆,陽性率高達 95-99% 。

 

Assy Principle: CyClone Ligase-free Cloning is based on generation of inserts with homologous ends to the linearized vector. (Quan and Tian (2009) Circular polymerase extension cloning of complex gene libraries and pathways. PLoS One 4:e6441)
Assy Flow: (1) Prepare vector and insert DNA, (2) Circularization reaction, (3) Transformation.

 

訂購資訊

Product Pack Size Cat. No.
CyClone Ligase-free Cloning Kit 20 reactions JB-PP-110S
100 reactions JB-PP-110L

 

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