Location: Home > Products > Our Products > 細胞分析 > 細胞生長與毒殺試驗 > DELFIA Cytotoxicity Kit

友善列印DELFIA Cytotoxicity Kit

由美商 PekrinElmer 公司所出品的「DELFIA Cytotoxicity Kit 」具有與傳統 Cr-51 釋放法 (Cr-51 Release Assay) 相比擬的偵測靈敏度,且實驗步驟類似,讓研究者輕鬆上手之外還能同時享有放射性實驗等級的偵測靈敏度、以及非放射性實驗所帶來的操作便利度與安全性!

 


The DELFIA Cytotoxicity assay exhibits strong specific release and its sensitivity correlates well with the 51Cr-release assay.
 
  • Sensitive – strong specific release; detect as few as 40 cells/well (96-well plate)

  • Time-saving – rapid cell loading; quick release of label; reduce assay time by as much as 50%

  • Convenient – stable fluorescent signal; longer product shelf-life than 51Cr

  • Non-Radioactive

 


Assay Principle of DELFIA EuTDA Cytotoxicity Reagents - The DELFIA Cytotoxicity assay takes advantage of a fluorescence enhancing ligand (BATDA) which crosses the cell membrane passively, enabling target cell loading that is both rapid and gentle. Once inside the cell, the ligand is immediately hydrolyzed by cellular esterases to generate a hydrophilic molecule (TDA) that can no longer penetrate the membrane. Cytolysis releases TDA into the supernatant to form a highly fluorescent lanthanide chelate with Europium; cytolysis levels are proportional to the amount of fluorescent signal produced.

 

訂購資訊

Product Pack Size Cat. No.
DELFIA EuTDA Cytotoxicity Reagents 960 assays PK-AD0116

 

首頁關於伯森最新消息 產品介紹代客服務聯絡我們 站內搜尋
© BLOSSOM BIOTECHNOLOGIES INC. All rights reserved.