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友善列印HCA ImagAmp Reagent Kits

由 PerkinElmer 公司使用其專利技術 "TSA™ (Tyramide Signal Amplification)" 所研發出的『HCA ImagAmp™』系列產品,可應用於高內涵篩選螢光偵測實驗 (high content screening, HCS),作為螢光訊號放大試劑使用。其技術原理是藉由 HRP 酵素催化標定有特定螢光分子的 Tyramide,使其大量且專一性地鍵結於偵測目標附近,進而達到訊號放大效果。 使用時不需大幅變動原本的實驗流程,僅需增加 HRP 酵素與 TSA 訊號放大試劑的使用步驟,過程約十分鐘,即可輕鬆享受靈敏度提升、測量時間縮短等優勢與便利。


產品特點:

  • 提高偵測靈敏度,增加偵測範圍。
  • 節省珍貴抗體,同時能維持偵測專一性。
  • 縮短偵測所需時間,提高實驗效率。
  • 確保訊號解析度,維持實驗再現性與可信度。

 

操作流程簡單快速:

產品效能:


HCA ImagAmp kits dramatically increase sensitivity with less primary Ab and reduced exposure times.
HeLa cells were treated with 1μg/mL Neocarzinostatin (NCS) for 1 hr before fixation and incubated with anti-phosphoH2AX at different concentrations. A) and B) Images show fluorophore channel only; nuclear counterstain was omitted for clarity. (A) secAb goat anti-mouse-HRP (1:1,000) and HCA ImagAmp 546, exposure time: 45 ms. (B) secAb goat anti-mouse-Alexa Fluor® 546, exposure time: 350 ms.

 

訂購資訊

Product Format* Cat. No.
HCA ImagAmp 488 Kit (Fluorescein) One 384-well microplate PK-NEL771001KT
HCA ImagAmp 546 Kit (Cyanine 3) One 384-well microplate PK-NEL774001KT
HCA ImagAmp 647 Kit (Cyanine 5) One 384-well microplate PK-NEL775001KT
*The format of the kit is based on 30 µl per well of Fluorophore Amplification Reagent Working Solution.

 

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