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找出您實驗的最佳轉染試劑

友善列印Ingenio® Electroporation Kits and Solutions

  • 良好的電穿孔效率!可有效地將 DNA / RNA / siRNA / miRNA 等核酸樣品送入真核細胞中,即使是難以轉染的幹細胞或初代細胞皆可成功適用。
  • 低細胞毒性!相較於他牌產品,Ingenio® 毒性更低,可保有更高的細胞存活率。
  • 適用於各種電穿孔設備機型!包含 Lonza-Amaxa®, Bio-Rad® or Harvard BTX® 等。
  • 價格更便宜!使用 Ingenio®,不僅可提供與 amaxa Nucleofector® Kits 相比擬的運送效率,還可讓您省下更多實驗經費。
  • 產品選擇彈性多元!具有完整試劑組合、以及獨立試劑、耗材配件等…多種產品規格可供選購。

 

Ingenio® Solution Provides Comparable Efficiency on amaxa Nucleofector® Device.Cells were electroporated in parallel with an EGFP reporter vector and assayed at 24 hours post-electroporation by flow cytometry. Two electroporators were used with different electroporation kits: the Ingenio® Electroporation Kit was used in the Gene Pulser Xcell™ Eukaryotic System (Bio-Rad) and the Amaxa Nucleofector II Device (Lonza); the Amaxa Nucleofector Kit V was used in the Amaxa Nucleofector II Device, all according to manufacturer's recommendations.

Efficient Plasmid DNA Delivery Using Ingenio® Solution with Amaxa Nucleofector Device. Ingenio® Electroporation Kits were used to transfect indicated cell types using Amaxa® Nucleofector® II Device. Cells were assayed at 24 hours by flow cytometry and reported as percentage of live cell population.

Ingenio® Outperforms Other Electroporation Solutions in Efficiency and Viability. Cells were electroporated in parallel with an EGFP reporter vector using either Ingenio® Electroporation Solution, PBS or the Gene Pulser® Electroporation Buffer (Bio-Rad) on the Gene Pulser Xcell™ Eukaryotic System. (A) EGFP expressing cells were identified 24 hours post-electroporation by flow cytometry and presented as a percentage of the live cell population. (B) Cells were assayed for viablility by propidium iodide staining and flow cytometry analysis. Experiments were performed in triplicate on three separate days and the data averaged.

Potent siRNA Knockdown of Transiently Expressed Firefly Luciferase Using the Ingenio® Electroporation Solution. siRNA and plasmid DNA were co-electroporated with the indicated cell lines with the Ingenio® electroporation solution in 0.2 cm cuvettes using either the Gene Pulser® (Bio-Rad) or the Amaxa Nucleofector® II (Lonza). 10µg/ml of plasmid encoding Firefly luciferase was co-electroporated with 250nM of either non-targeting siRNA control (Dharmacon Non-Targeting siRNA #1) or GL3 siRNA into Jurkat E6-1, HeLa and CHO-K1 cells. Twenty-four hours post electroporation, cells were harvested and assayed for luciferase activity. Data from independent experiments performed on different days were averaged then scaled to non-targeting siRNA control and are represented as a percentage of the control.

Pulse conditions used
:

(1) Amaxa Nucleofector® program:
a. Jurkat E6-1: X-001 with 10 x 106 cells/ml
b. HeLa: I-013 with 3 x 106 cells/ml
c. CHO-K1: U-023 with 5 x 106 cells/ml
  (2) Bio-Rad Gene Pulser® Exponential Decay setting:
a. Jurkat: 150V, 950µF with 10 x 106 cells/ml
b. HeLa : 130V, 950µF with 3 x 106 cells/ml
c. CHO-K1: 150V, 900µF with 5 x 106 cells/ml

High Efficiency Plasmid DNA Electroporation of Human Induced Pluripotent Stem (iPS) Cells using Ingenio®. The Ingenio® Electroporation Kit was used to transfect 2 x 106 iPS cells on the Amaxa® Nucleofector® II Device. Cells were electroporated with 8 µg ZsGreen expressing plasmid (Clontech) in 100 µl and plated in 6-well plates at 0.33 x 106 cells/well. Cells were visualized 24 hours post-transfection and imaged under 4X objective with an Olympus IX71® Inverted Microscope. Images are (A) phase contrast, and (B) green fluorescence. Cells were also assayed 24 hours post-transfection on an Accuri® Cytometer. The histogram (C) shows unelectroporated cells (black line) compared to cells electroporated with plasmid using the Ingenio® Electroporation Kit (green line).

 

訂購資訊

Product Pack Size Cat. No.
Ingenio® Electroporation Kit for Lonza-amaxa Nucleofector devices (0.2 cm cuvettes and cell droppers) 25 reactions MR-MIR50112
50 reactions MR-MIR50115
100 reactions MR-MIR50118
Ingenio® Electroporation Kits for all other electroporators, such as Bio-Rad and Harvard-BTX (0.4 cm cuvettes and cell droppers) 25 reactions MR-MIR50113
50 reactions MR-MIR50116
100 reactions MR-MIR50119
Ingenio® Electroporation Solution 25 reactions MR-MIR50111
50 reactions MR-MIR50114
100 reactions MR-MIR50117
Ingenio® Cell Droppers 25 MR-MIR50124
50 MR-MIR50125
Ingenio® Cuvettes for Lonza-amaxa Nucleofector devices 25 MR-MIR50120
50 MR-MIR50121
Ingenio® Cuvettes for all other electroporators, such as Bio-Rad and Harvard-BTX 25 MR-MIR50122
50 MR-MIR50123

 

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