QBlue Cell Viability Assay Kit

由美商 BioChain 公司所出品，偵測原理類似 Alamar Blue 染劑，可經由活細胞粒線體脫氫酶，將原本不具有螢光特性的反應試劑還原轉化為具有螢光特性的物質。反應靈敏，僅需 100 個細胞即可偵測得到。

Linear relationship between Cell Viability fluorescence and cell number. HEK293 cells w ere serial diluted and treated w ith Cell Viability Reagent in a 384-well plate. Fluorescence intensity was linear (R2 = 0.999) to the cell number. The detection limit estimated from the blank controls was 100 cells.   Dose-dependent cytotoxicity of saponin in HEK293 cells. HEK293 cells were plated at 15,000 cells per well (40 mL). Cells were treated with varying concentrations of saponin. After culture overnight, 5mL Cell Viability Reagent (solid circles) was added. AlamarBlue (open circles) was also tested for comparison. Following a three-hour incubation, fluorescence intensity was measured on a Molecular Devices LJL Analyst. Data represent mean ± SD (n = 3). The IC50 values of saponin for Cell Viability (signal: Background = 4.8-fold) and Alamar Blue (signal: Background = 3.3-fold) were 0.0016 wt% and 0.0017 wt%, respectively.

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