反轉錄-即時定量聚合酵素鏈鎖反應

One Step RT-qPCR
應用實驗	Probe-based RT-qPCR			Dye-based RT-qPCR
產品名稱	UltraPlex One-Step ToughMix	qScript XLT One-Step RT-qPCR ToughMix	qScript One-Step RT-qPCR	qScript One-Step SYBR Green RT-qPCR
作用機制	Hydrolysis Probes	Hydrolysis Probes	Hydrolysis Probes	SYBR Green I dye
靈敏度	++++	+++	++	++
Multiplex 偵測數量	>4	<4	<3	-
試劑組件數量	1	1	2	2
適用 RNA 樣本量	1 pg to 100 ng total RNA; 10 fg to 10 ng poly A(+) RNA; 10 to 1x10⁸ copies viral RNA
RT-qPCR 產物長度	<1 kb