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找出您實驗的最佳轉染試劑

友善列印TransIT®-2020

  • 高效率轉染試劑,即使是難以轉染的幹細胞或初代細胞皆可成功適用。
  • 適合各種 Plasmid DNA (包含一般基因表現載體、以及 siRNA, shRNA, miRNA, Pri-miRNA, Pre-miRNA, Virus 之表現載體) 轉染使用。
  • 品質優異,具有優於 FuGENE® HD、Lipofectamine™ 2000、Lipofectamine™ 2000 CD 的轉染效率。
  • 成份不含任何動物來源物質,大幅降低細胞污染的可能性。
Successfully Transfected Cells
Cell Lines Primary & Stem Cells
  • 2b4.11
  • A549
  • C3H/10T1/2
  • C6
  • CCD18Co
  • CHO.K1
  • CHO-K1
  • Colon38
  • COS7
  • Daoy
  • DB-TRG-05MG
  • DI-TNC1
  • HCT116
  • HEK-293
  • HEK-293T
  • HeLa
  • HepG2
  • HPTCs
  • INS-1
  • J774A.1
  • Jurkat
  • K562
  • KYSE-410
  • LS-180
  • MCF-7
  • MDA-MB-231
  • Monc-1
  • NCCIT
  • NE1
  • Neuro-2a
  • NIH/3T3
  • NRK52E
  • NSCLC cell line H522
  • PC-12
  • Phoenix Eco
  • RAW 264.7
  • RGC-5
  • Saos-2
  • SH-5YSY
  • SV40-Large T antigen rat aortic smooth muscle cells
  • T-98G
  • TE8
  • U2OS
  • Vero
  • Human ES Cell Derived Neural Progenitors
  • Human Mesenchymal Stem Cells
  • Human Airway Epithelial Cells
  • Human Astrocytes
  • Human Small Airway Epithelial Cells
  • Mouse Embryonic Fibroblasts (MEFs)
  • Mouse ES Cell Derived Cardiomyocytes

 

 
Superior Gene Expression in a Broad Spectrum of Cell Types. The indicated cell types were transfected in 96-well plates with a luciferase expression plasmid (0.1 µg/well) according to the manufacturer’s protocol. Reagent to DNA ratios were optimized for each cell type:  TransIT®-2020 (Mirus Bio, 2:1 or 3:1), FuGENE® HD (Roche, 3.5:1), Lipofectamine™ 2000 (Life Technologies, 1.5:1, 3:1 or 5:1).  Luciferase activity was measured 24 hours post-transfection. Values were normalized to TransIT®-2020 and presented as a percentage of luciferase expression.

TransIT®-2020 Reagent Efficiently Transfects Human Induced Pluripotent Stem (iPS) Cells. The TransIT®-2020 Transfection Reagent was used to transfect 0.5 x 106 iPS cells with a ZsGreen expressing plasmid (Clontech). Transfections were performed in 6-well plates using 7.5 µl of TransIT®-2020 Transfection Reagent to deliver 2.5 µg of DNA (3:1, reagent: DNA). Cells were visualized 24 hours post-transfection and imaged at 4X objective with an Olympus IX71® Inverted Microscope. Images are (A) phase contrast, and (B) green fluorescence. Cells were also assayed 24 hours post-transfection on an Accuri® Cytometer. The histogram (C) shows untransfected cells (black line) compared to cells transfected with plasmid using TransIT®-2020 (green line).

 

訂購資訊

Product Pack Size Number of Transfections in 6-well Plate Cat. No.
TransIT®-2020 Transfection Reagent 0.4 ml 50 MR-MIR5404
1.0 ml 125 MR-MIR5400
5 x 1.0 ml 625 MR-MIR5405
10 x 1.0 ml 1,250 MR-MIR5406

 

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