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找出您實驗的最佳轉染試劑

友善列印TransIT®-LT1

  • 低細胞毒性!可減輕試劑本身對細胞生長的影響性,降低實驗變異因素。
  • 良好的轉染效率!可有效地將 DNA 樣品轉染至多種細胞類型。
  • 節省時間與經費!單一試劑與實驗流程即可適用在多種細胞的轉染實驗,有效節省實驗花費與測試時間。
  • 應用範圍廣泛!可應用在單個或多個 plasmid DNA 的轉染實驗,應用領域包含:基因大量表現、siRNA / shRNA / miRNA 表現、病毒顆粒生產、報導基因等…。

 

產品效能:

  
The TransIT®-LT1 Reagent Exhibits Higher Expression and Lower Cellular Toxicity Compared to Other Transfection Reagents.
HepG2 cells were transfected with a luciferase expression plasmid using the designated reagents at the manufacturer’s recommended reagent-to-DNA ratio indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 µg of plasmid DNA per well. Luciferase expression (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as % cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph.
 
The TransIT®-LT1 Reagent Efficiently Delivers DNA to a Wide Variety of Cell Lines. Using the TransIT®-LT1 Transfection Reagent, cells were transfected with the pEGFP-C1 expression vector, and the percentage of EGFP expressing cells was determined 24-48 hours post-transfection.
              
High Efficiency Transfection of iCell® Cardiomyocytes using TransIT®-LT1 Transfection Reagent. iCell Cardiomyocytes were plated at 20,000 cells/well in a 96 well tissue culture plate coated with 0.1% gelatin. After allowing the cells to recover from thaw, cells were transfected with 100 ng/well of pMAXGFP (Lonza) using TransIT®-LT1 Transfection Reagent with a 2:1 reagent-to-DNA ratio according to the manufacturer's instructions. Fluorescent images were taken 3 days post transfection using a Olympus IX71® inverted microscope.

 

訂購資訊

Product Pack Size Number of Transfections in 6-well Plate Cat. No.
TransIT®-LT1 Transfection Reagent 0.4 ml 50 MR-MIR2304
1.0 ml 125 MR-MIR2300
5 x 1.0 ml 625 MR-MIR2305
10 x 1.0 ml 1,250 MR-MIR2306

 

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