產品介紹蛋白質體學研究Western Blot 實驗工具Western Blot Enhancing Kit

Western Blot Enhancing Kit

Attoglow Western Blot Analysis Kit is an ultra-sensitive chemiluminescent Western blot assay. It detects protein at attogram (10-18 g) or yoctomole (10-24 M) levels. One ng of purified Rabbit muscle GAPDH was serially diluted to 1 attogram and electrophoresis was performed. The gel was transferred to a PVDF membrane, and then cut in the middle. The right section was treated with Millennium Enhancer, and the left was untreated. Immunoblotting was done using a primary antibody (clone 6C5) and HRP-conjugated rabbit anti-mouse antibody, and chemiluminescent substrates were applied. A film exposed for two minutes is shown above. 10-11 g of antigen without treatment and 10-14 g of GAPDH with treatment gave similar signal strengths, indicating a 1000-fold enhancement*.
*Fold enhancement varied from a few- to a 1000-fold depending on the characteristics of antibodies and antigen used.

訂購資訊

Product Name Pack Size Cat. No.
Western Blot Enhancing Kit for 1200 cm² membrane BC-K3172120
for 2500 cm² membrane BC-K3172250
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