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友善列印µ-Slide Membrane ibiPore Flow

  • 具備兩條可培養細胞的通道,通道交疊處採多孔玻璃膜設計,能將傳統使用 Transwell (Boyden chamber) 進行的各式 Transmigration & Transport 試驗轉移至流體環境下進行,模擬循環系統,更貼近生理環境!
  • 搭配幫浦 (例如 ibidi Pump) 不同剪切應力設定,可模擬生理上不同流體環境,如動脈、靜脈、肺泡等。
  • 玻璃膜具極佳光學性質,可輕易獲得清晰顯微鏡成像。
  • 針對不同細胞、不同應用,提供多種玻璃膜孔徑大小可選。
  • 符合標準載玻片規格 (75.5 x 25.5 mm),廣泛適用各類倒立式顯微鏡。

產品用途

  • 通過多孔膜的細胞遷移 (Transmigration)、趨化 (Chemotaxis)、侵入 (Invasion) 試驗。
  • 肺泡細胞或皮膚細胞微環境模擬。
  • 細胞極化試驗 (Apical-basal cell polarity assays)。
  • 細胞共同培養 (Co-culture)、血腦障壁 (Blood brain barrier) 模擬等等。

 

Specifications µ-Slide Specifications ibiPore Membrane
Total coating area 4.50 cm² Material SiO₂ (glass)

Volume of main channel 50 µl Thickness 0.3 µm (300 nm)
Height of main channel 0.4 mm Membrane size 2 mm x 2 mm
Length of main channel 25 mm
Available variations Pore Size
0.5 µm
3 µm
5 µm
8 µm
Porosity
20%
5%
5%
5%
Width of main channel 5 mm  
Growth area, main channel 1.25 cm²
Volume of upper channel  55 µl
Height over membrane 1.3 mm

 

Recommended Pore Sizes and Porosities for Different Applications
Type of Membrane
Applications Example Cell Types
0.5 µm pores, 
high porosity (20%)
Permeability and transport studies,
co-culture models
Lung cells, epithelial cells
3.0 µm pores, 
low porosity (5%)
Transendothelial migration Leukocytes (e.g., neutrophils), lymphocytes (e.g., T cells or B cells)
5.0 µm pores, 
low porosity (5%)
Invasion, migration Monocytes, macrophages, lymphocytes (e.g., T cells or B cells)
8.0 µm pores, 
low porosity (5%)
Invasion, migration Tumor and cancer cells, endothelial and epithelial cells, fibroblasts, osteoblasts, melanoma cells, glioma cells
*The bigger the cell – the bigger the recommended pore size.

 

 

訂購資訊

Product Pack Size Cat. No.
µ-Slide Membrane ibiPore 0.5µm/20% Flow ibiTreat: #1.5 polymer coverslip, tissue culture treated, 0.5 µm porous glass membrane, 20% porosity, sterilized 10 pcs. IB-85116
µ-Slide Membrane ibiPore 3.0µm/5% Flow ibiTreat: #1.5 polymer coverslip, tissue culture treated, 3.0 µm porous glass membrane, 5% porosity, sterilized 10 pcs. IB-85126
µ-Slide Membrane ibiPore 5.0µm/5% Flow ibiTreat: #1.5 polymer coverslip, tissue culture treated, 5.0 µm porous glass membrane, 5% porosity, sterilized 10 pcs. IB-85136
µ-Slide Membrane ibiPore 8.0µm/5% Flow ibiTreat: #1.5 polymer coverslip, tissue culture treated, 8.0 µm porous glass membrane, 5% porosity, sterilized 10 pcs. IB-85146
µ-Slide Membrane ibiPore 0.5µm/20% Flow ibiTreat Sample Pack: #1.5 polymer coverslip, tissue culture treated, 0.5 µm porous glass membrane, 20% porosity, sterilized 2 pcs. IB-85116-S
µ-Slide Membrane ibiPore 3.0µm/5% Flow ibiTreat Sample Pack: #1.5 polymer coverslip, tissue culture treated, 3.0 µm porous glass membrane, 5% porosity, sterilized 2 pcs. IB-85126-S
µ-Slide Membrane ibiPore 5.0µm/5% Flow ibiTreat Sample Pack: #1.5 polymer coverslip, tissue culture treated, 5.0 µm porous glass membrane, 5% porosity, sterilized 2 pcs. IB-85136-S
µ-Slide Membrane ibiPore 8.0µm/5% Flow ibiTreat Sample Pack: #1.5 polymer coverslip, tissue culture treated, 8.0 µm porous glass membrane, 5% porosity, sterilized
2 pcs. IB-85146-S

 

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