AP 酵素呈色受質

BCIP/NBT

由 Revvity 公司 (源自 PerkinElmer 生命科學和診斷部門) 所出品，與 AP 酵素作用反應後可產生深紫色沈澱。

Comparison of 4CN Plus and BCIP/NBT detection methods. Dilutions of bovine α-tubulin (starting at 800 ng) were electrophoresed and electroblotted onto PolyScreen PVDF Transfer Membrane. Western blot detection was carried out using anti-tubulin antibody, either goat anti-mouse HRP or goat anti-mouse AP. Stained blots were visualized on a commercially available imaging system.

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FastRed Substrate Kit

FastRed/Napthol AS-Mx 是目前免疫組織化學染色實驗 (IHC) 或原位雜交實驗 (ISH) 最常使用到的 AP 酵素呈色試劑。由美商 BioChain 公司所出品的「FastRed Substrate Kit」，產品內含 A 與 B 兩種 20 倍濃縮試劑，以水稀釋混合後即可馬上使用，非常簡單便利。與 AP 酵素作用反應後可產生粉紅色沈澱。

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