Attoglow ECL 冷光試劑

由 BioChain 公司所出品的「Attoglow」系列 ECL 冷光試劑可經由 HRP (horseradish peroxidase) 酵素作用反應產生化學冷光，適用於 Western Blot 實驗，偵測靈敏度可達 Attogram (10^-18 g) 等級。其產品優勢包含：

靈敏度極佳：獨家 Millenium Enhancer 試劑可增強抗原與抗體之間的親合性，進而提高偵測靈敏度。
高效便利：內含 Millenium Enhancer、冷光試劑、二級抗體、阻斷液 (blocking solution) 等完整試劑組件，使用更便利。

Attoglow Western Blot Analysis Kit is an ultra-sensitive chemiluminescent Western blot assay. It detects protein at attogram (10^-18 g) or yoctomole (10^-24 M) levels. One ng of purified Rabbit muscle GAPDH was serially diluted to 1 attogram and electrophoresis was performed. The gel was transferred to a PVDF membrane, and then cut in the middle. The right section was treated with Millennium Enhancer, and the left was untreated. Immunoblotting was done using a primary antibody (clone 6C5) and HRP-conjugated rabbit anti-mouse antibody, and chemiluminescent substrates were applied. A film exposed for two minutes is shown above. 10^-11 g of antigen without treatment and 10^-14 g of GAPDH with treatment gave similar signal strengths, indicating a 1000-fold enhancement*.
*Fold enhancement varied from a few- to a 1000-fold depending on the characteristics of antibodies and antigen used.

訂購資訊

Product Name	Pack Size	Cat. No.
Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti mouse secondary antibody	for 1200 cm² membrane	BC-K3171120-I
	for 2500 cm² membrane	BC-K3171250-I
Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti rabbit secondary antibody	for 1200 cm² membrane	BC-K3171120-II
	for 2500 cm² membrane	BC-K3171250-II
Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti chicken secondary antibodycm² membrane	for 1200 cm² membrane	BC-K3171120-III
	for 2500 cm² membrane	BC-K3171250-III
Attoglow Western Blot Analysis Kit- with Millennium Enhancer, Anti goat secondary antibody	for 1200 cm² membrane	BC- K3171120-IV
	for 2500 cm² membrane	BC- K3171250-IV