CytoSMART Lux3 BR 活細胞即時影像監測系統

Fig. 1 | Digital images suitable for scientific publications. The CytoSMART Lux3 BR is ideal for capturing crisp brightfield images and videos of living cells. The image size of 2072×2072 pixels combined with the 1.45×1.45 mm field of view provide a resolution of 0.7 µm/pixel. Even at the commonly required image resolution of 300 dpi for printed scientific publications, these images can fill the entire page width if desired, without compromising the image quality.

Fig. 2 | HeLa cells were seeded in the ibidi µ-Slide Chemotaxis, with either FBS gradient or constant FBS concentration. Cultures were monitored for 24 h using the CytoSMART Lux3 BR Duo Kit, with an imaging interval of 5 min. HeLa cells cover a larger distance in constant FBS concentration, but prefer to migrate towards a higher FBS concentration when exposed to a gradient. (A) Raw images of HeLa cells made using CytoSMART Lux3 BR Duo Kit, single cell detection (purple) and path detection (yellow) with FIJI-plugin TrackMate. (B) Chemotactic displacements of HeLa cells visualized using FIJI-plugin Chemotaxis and Migration Tool, with longitudinal and lateral displacements defined with respect to the FBS gradient.