DELFIA Cell Proliferation Kit
全新改版的 DELFIA Cell Proliferation Kit 採用「新款 Eu-labeled Anti-BrdU 抗體」，不但訊號-背景比值增加為舊款產品的 3 倍，抗體也由原本的粉末狀改為更加方便使用的液態型式，讓產品性能與操作便利度同時向上提升！DELFIA Cell Proliferation Kit 產品特點包含：
- 高靈敏度：可達 100 cells/well。
- 高便利性：貼附和懸浮型細胞均可適用。
- 高安全性：非放射線方法，無放射線汙染疑慮。
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偵測原理
Assay Principle of DELFIA Cell Proliferation Kit. The DELFIA cell proliferation assay is a time-resolved fluoroimmunoassay based on the incorporation of BrdU into newly synthesized DNA strands of proliferating cells cultured in microliter plates. Incorporated BrdU is detected using a europium labelled monoclonal antibody. To allow antibody detection cells are fixed and DNA denatured using Fix Solution. Unbound antibody is washed away and DELFIA Inducer is added to dissociate europium ions from the labelled antibody into solution, where they form highly fluorescent chelates with components of the DELFIA Inducer. The fluorescence measured is proportional to the DNA synthesis in the cell population of each well.
訂購資訊
| Product Name | Pack Size | Cat. No. |
|---|---|---|
| DELFIA Cell Proliferation Kit | 10 x 96 wells | PK-AD0200 |
