DELFIA Cytotoxicity Kit

Assay Principle of DELFIA EuTDA Cytotoxicity Reagents. The DELFIA Cytotoxicity assay takes advantage of a fluorescence enhancing ligand (BATDA) which crosses the cell membrane passively, enabling target cell loading that is both rapid and gentle. Once inside the cell, the ligand is immediately hydrolyzed by cellular esterases to generate a hydrophilic molecule (TDA) that can no longer penetrate the membrane. Cytolysis releases TDA into the supernatant to form a highly fluorescent lanthanide chelate with Europium; cytolysis levels are proportional to the amount of fluorescent signal produced.