ExpressPlex™ 2.0 Library Prep Kit

產品特色

Express 2.0： 搭載高效 TnX transposase，簡化建庫流程，一步驟完成上機前準備！
TnX transposase： 降低片段化時的序列偏好性，提升文庫均勻度。
簡化操作： 僅需 100 分鐘完成建庫，實際動手操作不到 30 分鐘，即可完成 DNA 片段化、barcode 連接與文庫放大。
支援高通量： 適配 96 與 384 孔板操作，最多可處理 6144 個樣本，並內建 自動校正(auto-normalization) 功能，確保上機樣本均一性。

更多詳細資訊歡迎洽詢 seqWell 台灣代理伯森生技。

Library preparation for pUC19 and pMal-c67 was conducted using ExpressPlex 2.0 kit and a Tn5-based competitor kit using standard manufacturers’ protocols. Libraries were sequenced using an Illumina MiSeq.

ExpressPlex 2.0 One-step Library Prep (96-well format)

ExpressPlex 2.0 One-step Library Prep (384-well format)

產品規格

ExpressPlex 2.0 Specifications
Primary Applications	Plasmid & amplicon sequencing, and microbial whole genome sequencing (WGS)
Sample Input types	2Purified plasmid DNA, RCA-amplified DNA, amplicons >350bp, colony PCR amplicons, microbial genomic DNA
Transposase	TnX – Next generation engineered transposase
Kit format	96-well 384-well Custom dispense
DNA Input Recommended	96-well format: 1-40 ng for plasmid & amplicon seq 10-40 ng for microbial WGS 384-well format: 0.5 – 20 ng for plasmid & amplicon seq
Total Library Prep Time	100 minutes (30 minutes hands-on time)
Indexing Method	Combinatorial Dual Indexing
Number of Unique Index Combinations	Up to 6144
Batch Size	8-96 samples (96-well format) 384 samples (384-well format)
Output Fragment Range*	400 – 1,200bp
Number of PCR Cycles	12 cycles for plasmids 15 cycles for amplicons 12 cycles for microbial WGS
Sequencer Compatibility	All Illumina sequencing platforms Compatible with Complete Genomics platforms or Element Biosciences AVITI™ using conversion kits for Illumina libraries

* Fragment size will depend on magnetic bead cleanup ratios used.

產品資訊

Product	Total sample	Index	Cat. No.
ExpressPlex 2.0 (96-well) Library Preparation Kit 4 x 96 Reactions - Set 1000	384	CDI	301176
ExpressPlex 2.0 (96-well) Library Preparation Kit 4 x 96 Reactions - Set 2000	384	CDI	301177
ExpressPlex 2.0 (96-well) Library Preparation Kit 4 x 96 Reactions - Set 3000	384	CDI	301178
ExpressPlex 2.0 (96-well) Library Preparation Kit 4 x 96 Reactions - Set 4000	384	CDI	301179
ExpressPlex 2.0 (96-well) Library Preparation Kit 1 x 96 Reactions - Any Index	384	CDI	301170
ExpressPlex 2.0 (384-well) Library Preparation Kit 4 x 384 Reactions - Set A	1536	CDI	301141
ExpressPlex 2.0 (384-well) Library Preparation Kit 4 x 384 Reactions - Set B	1536	CDI	301142
ExpressPlex 2.0 (384-well) Library Preparation Kit 4 x 384 Reactions - Set C	1536	CDI	301143
ExpressPlex 2.0 (384-well) Library Preparation Kit 4 x 384 Reactions - Set D	1536	CDI	301144
ExpressPlex 2.0 (384-well) Library Preparation Kit 1 x 384 Reactions - Any Index	1536	CDI	301152