HCA ImagAmp™ Reagent Kits
由 Revvity 公司使用 Tyramide Signal Amplification (TSA) 技術所研發出的「HCA ImagAmp™」系列產品，可應用於高內涵篩選 (high content screening, HCS) 螢光偵測實驗，作為螢光訊號放大試劑使用。其技術原理是藉由 HRP 酵素催化標定有特定螢光分子的 Tyramide，使其大量且專一性地鍵結於偵測目標附近，進而達到訊號放大效果。使用時不需大幅變動原本的實驗流程，僅需增加 HRP 酵素與 TSA 訊號放大試劑的使用步驟，過程約十分鐘，即可輕鬆享受靈敏度提升、測量時間縮短等優勢與便利。
產品特點
- 提高偵測靈敏度，增加偵測範圍。
- 節省珍貴抗體，同時能維持偵測專一性。
- 縮短偵測所需時間，提高實驗效率。
- 確保訊號解析度，維持實驗再現性與可信度。
操作流程簡單快速
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產品效能
HCA ImagAmp kits dramatically increase sensitivity with less primary Ab and reduced exposure times. HeLa cells were treated with 1μg/mL Neocarzinostatin (NCS) for 1 hr before fixation and incubated with anti-phosphoH2AX at different concentrations. (A) and (B) Images show fluorophore channel only; nuclear counterstain was omitted for clarity. (A) secAb goat anti-mouse-HRP (1:1,000) and HCA ImagAmp 546, exposure time: 45 ms. (B) secAb goat anti-mouse-Alexa Fluor® 546, exposure time: 350 ms.
訂購資訊
| Product Name | Format* | Cat. No. |
|---|---|---|
| HCA ImagAmp 488 Kit (Fluorescein) | One 384-well microplate | PK-NEL771001KT |
| HCA ImagAmp 546 Kit (Cyanine 3) | One 384-well microplate | PK-NEL774001KT |
| HCA ImagAmp 647 Kit (Cyanine 5) | One 384-well microplate | PK-NEL775001KT |
*The format of the kit is based on 30 µl per well of Fluorophore Amplification Reagent Working Solution.
