Lentivirus Packaging Mix Powered by MISSION® Genomics
- 高生產效率:搭配 TransIT®-Lenti 使用,具感染力的慢病毒 (lentivirus) 力價可高達 10⁸。
- 操作簡易:只需一管即含有組裝 lentivirus 病毒顆粒所需的套膜 (envelope)、蛋白質膜 (capsid) 蛋白元件、以及負責將目標基因嵌入宿主染色體所必須的反轉錄酶 (reverse transcriptase) 和嵌合酶 (integrase)。
歡迎與我們聯繫索取更多 Lentivirus Packaging Mix Powered by MISSION® Genomics 產品資訊與文獻。
產品效能
Fig. 1 | Up to 100-fold Increase in Functional Lentivirus Titers. Adherent 293T/17 cells were transfected in a 6-well plate with the pLKO.1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus Packaging Mix Powered by MISSION® Genomics or pLenti6.2-GW/EmGFP transfer vector and the ViraPower™ Lentiviral Packaging Mix (1:1 ration, 2 µg/well) with the following reagents: TransIT®-Lenti (3:1, vol:wt), Lipofectamine® 2000 (3:1), or Lipofectamine® 3000 (3:1:1). The supernatant was harvested, filtered (0.45 µm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 µg/ml TransduceIT™ and GFP expression was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Error bars represent triplicate transfection complexes titered individually. Functional titers were calculated using virus dilutions with less than 20% GFP positive cells.
Fig. 2 | Transfer Vector Selection Greatly Affects Functional Lentivirus Titers. Adherent 293T/17 cells were transfected in a 6-well plate with one of three transfer vectors: MISSION® pLKO.1-puro-CMV-TurboGFP™, ViraPower™ pLenti6.2-GW/EmGFP, or Precision Lenti-ORF™ RFP and the Lentivirus Packaging Mix powered by MISSION® Genomics (1:1 ratio, 2 µg/well) using the TransIT®-Lenti Transfection Reagent (3:1, vol:wt). The supernatant was harvested, filtered (0.45 µm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 µg/ml TransduceIT™ and GFP expression was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Error bars represent triplicate transfection complexes titered individually. Functional titers were calculated using virus dilutions with less than 20% GFP positive cells.
Fig. 3 | Plasmid Maps. The Lentivirus Packaging Mix Powered by MISSION® Genomics is an optimized formulation of two plasmids expressing the structural and regulatory (gag, pol and rev) and viral envelope (VSV-G) proteins required for producing high titer lentivirus with broad viral tropism.
訂購資訊
Product Name | Pack Size | Cat. No. |
---|---|---|
Lentivirus Packaging Mix Powered by MISSION® Genomics Includes: Plasmid packaging mix only; Does Not include transfection reagent |
5 rxn | MR-MIR6630 |
34 rxn | MR-MIR6640 |