Lite™ 系列報導基因偵測套組

Firefly luciferase from the North American firefly (Photinus pyralis) catalyzes the oxidation of the fireflyspecific substrate D-luciferin to produce light. Light emission results from multi-step reactions. The initial reversible reaction step is the activation of D-luciferin in the presence of ATP, Mg²⁺ and luciferase enzyme which leads to enzyme bound adenyl-luciferin with the elimination of inorganic pyrophosphate (PPi). Subsequent reaction steps involve the oxidation of adenyl-luciferin with molecular oxygen via adenyl-oxyluciferin to yield AMP, CO₂ and oxyluciferin. The oxyluciferin is generated in an electronically excited state which emits light upon transition to the ground state. Renilla luciferase catalyzes the oxidation of the substrate coelenterazine (coelenterate luciferin) to produce light. However, unlike the firefly reaction, Renilla luciferase luminescence does not require ATP or magnesium.