Revio™ 高精準長讀取定序系統

PacBio Revio™ 高精準長讀取定序系統，專為大規模 HiFi 定序需求而設計。搭配最新 SPRQ-Nx chemistry，進一步提升定序通量、降低樣本需求與定序成本，讓高精準長讀取定序更適合大規模人類全基因體與多元研究應用。

更高通量：Revio 系統可同時運行最多 4 張 SMRT Cell，搭配 SPRQ-Nx chemistry，每次運行最高可產出 480 Gb HiFi reads，支援大規模全基因體定序需求。
更低樣本需求與更高使用彈性：每次 acquisition 僅需 500 ng DNA，並支援 SMRT Cell 重複使用，每張 SMRT Cell 最多可進行 3 次 acquisition，提升珍貴或有限樣本的應用彈性與耗材使用效率。
豐富的基因體資訊：HiFi reads 兼具長讀長與高準確度，可分析 SNV、INDEL、結構變異、重複序列與 haplotype phasing，並可於定序資料中偵測 5mC、5hmC 與 6mA DNA 修飾資訊。
快速靈活：支援多種定序時間，開始定序後即可使用工作載台安排下次上機，可依樣本與研究需求彈性安排定序流程，並透過自動化 loading、SMRT Cell washing 與機上資料分析功能，簡化日常操作。

更多詳細資訊歡迎洽詢 PacBio 台灣代理伯森生技。

	Revio (HiFi sequencing)	SBS sequencing	Nanopore sequencing
Read length	15–20 kb⁵	2x150 bp	10–100 kb
Read accuracy	99.95% (Q33)¹	99.92% (Q31)³	98.90% (Q19)⁴
Run time	24 hours²	44 hours	72 hours
Yield	120-480 Gb²	2,400–3,000 Gb	50–110 Gb
Coverage	Unbiased	Reduced at low and high (GC)	Reduced in low-complexity runs
Genome completeness⁶	✓	X	✓
Variant calling — SNVs, Indels, SVs⁵	✓	X	X
Areas of high homology	✓	X	X
Phasing	✓	X	✓
Methylation	Every run	Via library prep	Every run

Read accuracy PacBio HiFi: precisionFDA Truth Challenge V2
HiFi yield specification based on HG002/GM24385 human DNA extracted with Nanobind CBB kit and prepared with SMRTbell prep kit 3.0. Run time specification is for the sequencing reaction. HiFi yield is dependent on library fragment size. Yield is typically lower for shorter libraries.
Illumina: HG002 2×150 bp NovaSeq library, precisionFDA Truth Challenge V2
ONT: Q20+ chemistry (R10.4, Kit 12), Oct 2021 GM24385 Q20+ Simplex dataset release
Shafin Nat Methods 2021 ( https://doi.org/10.1038/s41592-021-01299-w )
Cheng 2021 Nat Methods ( https://doi.org/10.1038/s41592-020-01056-5 ) and Shafin 2020 Nat Biotech ( https://doi.org/10.1038/s41587-020-0503-6 )

產品規格

Library	Run time^a	Q30+ bases	HiFi yield per SMRT Cell^b	Methylation
0.5-5 kb^d	12 hours	95%	6-8 million reads	5mC and 5hmC at CpG sites and 6mA for native DNA^c
5-10 kb	24 hours	90%	35-70 Gb
10-15 kb			70-100 Gb
15-20 kb			100-120 Gb
20-25 kb	30 hours	85%	100-120 Gb

Run time refers to the data collection step, which determines the time between processing SMRT Cells.
HiFi yield is dependent on library quality and sequencing preparation procedures. Specified yield is based on high-quality samples prepared following best practices.
The Revio 6mA caller is designed to detect methylation in the context of the Fiber-seq chromatin assay.
Libraries 0.5-5kb are supported only with single acquisition Revio SMRT Cells with either SPRQ or SPRQ-Nx chemistry.

Library	Application	Per Revio SMRT Cell	Per year^a	Expected coverage^b
0.5-5 kb^c	Amplicon sequencing	> 1,000	> 2.5 million	50x
5-10 kb	Microbial genome	384	480,000	30x
5-15 kb	PureTarget panels	96	96,000	>100x mean per target
5-10 kb	Ampli-Fi human genome	1	1,250	20x
15-20 kb	Human genome	2	2,500	20x
15-20 kb	Human methylation profiling	8	10,400	5x
15-20 kb	Transcriptome with Kinnex full-length RNA kit	6	7,500	10 million reads

Annual throughput is based on 2,500 Revio SMRT Cells for 12 hour runs; 1,250 for 24 hour runs. Adaptive loading – which increases the consistency of yield per SMRT Cell – adds about 4 hours to run time, affecting the maximum number of SMRT Cells per year.
Expected coverages are estimates.
0.5-5kb amplicon sequencing performance with SPRQ-Nx chemistry based on single-use SMRT Cells

Instrument specifications
Operating environment	Temperature: 19–25°C Humidity: 20–80% relative humidity, non-condensing Ventilation: 13,000 BTU/hr (3,800 W)
Dimensions	W × D × H: 92.7 cm × 91.4 cm × 174.5 cm Weight: 465 kg Floor space: 243.8 cm × 138.5 cm
Electrical power	200–240 VAC at 50-60 Hz, max 5 kVA/4.8 kW/24A
Compute	Network connection: 1 GbE or 10 GbE, copper Output file format: hifi_reads.bam, ≈60 GB each, up to 78 TB per year