SUMOylate and detect a broad range of target proteins. Western Blot of SUMOylation Assays for RANGAP1 control target and SP100/p53 target proteins. Assays set-up and run as described in “Assay Protocol”. SUMOylated proteins were detected by Western Blotting on SUMOylation assays containing A: RANGAP1 (Prod. No. BML-KW9755), Figure B: p53 (Prod. No. BML-FW9370) and Figure C: SP100 (Prod. No. BML-UW9825) target proteins with 1: SUMO1 (Prod. No. BML-KW9195), 2: SUMO2 (Prod. No. BML-KW9205) and 3: SUMO3 (Prod. No. BML-KW9215) substrates using the appropriate SUMO antibody (1: Prod. No. BML-PW9460, 2/3: Prod. No. BML- PW9465) as described in “Analysis by Western Blotting”. |