阻斷液

Fig. 1 | Western Blotting: Only the combination of Blocking Solution and LowCross-Buffer^® (as dilution buffer for primary and secondary antibodies) leads to a clear result. Detection of CaMKII in lysate from heart and brain tissues of C57Bl/6 mice. Monoclonal Anti CaMKII was used as primary antibody and a polyclonal HRP conugate as secondary antibody. Data from Dr. Tronhung Quang, Dr. Rainer Klocke and Dr. Sigrid Nikol, university hospital of Münster, cardiology

Fig. 2 | ELISA (competitive): Decrease of the CV (with n=96, measured at the maximum B0). The Blocking Solution impressively lowered the coefficient of variation as shown in this successful assay validation of a competitive ELISA. Data from Dr. P. Rauch, CANDOR Bioscience GmbH

Fig. 3 | Immunohistochemistry: Using Blocking Solution leads to a strong reduction of unwanted two-dimensional background. Detection of nestin on cytospin-preparation of the neuroblastoma cell line SK-N-LO by ABC-immunocytochemical staining with alkaline phosphatase. After correct staining of cytoplasmatical nestin also the haematoxylin counterstain of the cell nuclei is not problematical any longer for the interpretation of results. Data from Dr. Astrid Voigt, paediatric clinic, university of Jena

Fig. 4 | Immunohistochemistry: The increase can be tracked correctly by using CANDOR The Blocking Solution. Detection of osteocalcin in osteoblast culture. A fresh osteoblast culture shows, on the first day, no or very weak expression of the extracellular matrix protein osteocalcin. Using Blocking Solution the situation is presented correctly (shown left side, first picture). Standard blocking with BSA leads to a false positive result (right side). With increasing duration of cell culture, extra-cellular matrix and osteocalcin is building up. The cultures are stained intensively. Data from PH Dr. H.-P. Wiesmann, group tissue engineering and biomaterials, clinic for oral and maxillofacial surgery, university of Münster