TransIT®-EE
- 適合 Plasmid DNA 的活體核酸運送 (轉染) 實驗。
- 效率佳,不需搭配使用 Polymer 聚合體即可擁有高於 TransIT®-QR 兩至三倍的運送效率。
- 可搭配 pLIVE® Vector 使用,使目標基因專一性地於肝臟中大量表現。
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產品效能
Fig. 1 | TransIT®-EE Solution Leads to Enhanced Luciferase Expression in the Liver. TransIT®-QR or TransIT®-EE Delivery Solutions were used to deliver 10 µg of a luciferase expression vector to mice using the hydrodynamic delivery tail vein injection procedure. Twenty-four hours post-injection the livers were harvested and assayed for luciferase activity.
Fig. 2 | TransIT®-EE Solution Leads to Enhanced Luciferase Expression in a Variety of Organs. TransIT®-QR or TransIT®-EE Delivery Solutions were used to deliver 10 µg of pCI-Luc (CMV promoter-driven luciferase expression vector) to mice using the hydrodynamic tail vein injection procedure. Twenty-four hours post-injection the organs were harvested and assayed for luciferase activity.
Following gene delivery via a tail vein injection, the highest level of transgene expression is found in the liver, with reduced levels of expression found in the spleen, lung, heart and kidneys.
Following gene delivery via a tail vein injection, the highest level of transgene expression is found in the liver, with reduced levels of expression found in the spleen, lung, heart and kidneys.
訂購資訊
Product Name | Pack Size | Cat. No. |
---|---|---|
TransIT®-EE Delivery Solution | 40 injections | MR-MIR5340 |
pLIVE® Vector | 20 µg | MR-MIR5420 |
pLIVE® Vector/lacZ Control Vector Kit | 20 µg of each vector | MR-MIR5520 |
pLIVE® Vector/SEAP Control Vector Kit | 20 µg of each vector | MR-MIR5620 |
pLIVE® Vector Complete System | 20 µg of each (all 3 vectors) | MR-MIR5320 |