UBIQAPTURE-Q kit

由國際知名大廠 Enzo Life Sciences 所出品的「UBIQAPTURE-Q^® kit」可應用於純化 Cell lysates 和 Tissue extracts 中的 Ubiquitinylated proteins。採用離心式純化步驟，操作簡單便利！其他產品特點包含：

• 無論是 Mono-, Multi-, Poly-ubiquitinylated proteins 皆能被有效純化，結合力 (binding capacity) >1 µg ubiquitinylated protein/20 μl matrix suspension。
• 適合 Human、Mouse、Rat、Yeast 來源的 cell/tissue lysates 樣本純化，亦可適用於 in vitro ubiquitinylation 的反應產物純化。
• 試劑隨附 Ubiquitinylated-protein lysates、HRP-conjugated anti-ubiquitin Ab，可分別作為實驗對照組以及 Western Blot 分析使用。
• 純化產物仍可保有生物活性（需搭配適當試劑與流程方法，歡迎索取詳細技術資料）。

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產品效能

Western Blot analysis of ubiquitin enrichment of lysate derived ubiquitinylated proteins. Ubiquitin enrichment experiment set-up and run as described in UBIQAPTURE-Q® kit (Prod. No. BML-UW8995A). Ubiquitin-protein conjugates present in starting material, unbound fraction and elution fraction were detected by western blotting using the provided ubiquitin-conjugate specific HRP-linked antibody (Prod. No. ENZ-ABS840HRP) at a dilution of 1:1000. Key: SM = Starting Material, UF = Unbound Fraction, WA = Wash Fraction, and EL = Elution Fraction.

訂購資訊