Ubiquitinylation kit
由國際知名大廠 Enzo Life Sciences 所出品的「Ubiquitinylation kit」內含 Ubiquitin (biotinylated)、Ubiquitin activating enzyme (E1) (His6-tagged)、以及 Ubiquitin conjugating enzyme (E2) (His6-tagged) 等試劑，可作用產生 E2-ubiquitin thioester，應用於下列分析實驗中：
- 製造 Ubiquitinylated Protein：搭配適當的 Ubiquitin ligating enzyme (E3) 使用，可應用於目標蛋白的泛素化反應 (Ubiquitinylation)。E3 酵素來源可為重組蛋白、純化產物或 Cell lysates。
- 測試參與目標蛋白泛素化反應的最佳 E2 酵素：由於本產品提供多達 11 種不同的 Ubiquitin conjugating enzyme (E2) 試劑，因此可應用於測試參與目標蛋白泛素化反應的最佳 E2 酵素為何。
- E3 酵素活性分析：可建立 in vitro 分析平台找尋可作用於特定目標蛋白的 E3 酵素分子。
歡迎與我們聯繫索取更多 Ubiquitinylation kit 產品資訊與優惠活動訊息。
產品效能
Western Blot of Thioester Assays (TE +ve/-ve controls) for all E2 conjugating enzymes provided. Procedures as described in “Assay Protocol” section. Biotinylated-ubiquitin-enzyme conjugates were detected by Western Blotting on thioester assays containing A: UbcH1 (Prod. No. BML-UW9020), B: UbcH2 (Prod. No. BML-UW9025), C: UbcH3 (Prod. No. BML-UW8730), D: UbcH5a (Prod. No. BML-UW9050), E: UbcH5b (Prod. No. BML-UW9060), F: UbcH5c (Prod. No. BML-UW9070), G: UbcH6 (Prod. No. BML-UW8710), H: UbcH7 (Prod. No. BML-UW9080), I: UbcH8 (Prod. No. BML-UW9135), J: UbcH10 (Prod. No. BML-UW0960), K: Ubc13/MMS2 (Prod. No. BML-UW9565) respectively, using Streptavidin-HRP detection system as described in “Analysis by Western Blotting” section. M: Biotinylated SDS molecular weight markers (Sigma, SDS-6B) from bottom: 20.1, 29.0, 39.8, 58.1kDa.
訂購資訊
| Product Name | Pack Size | Cat. No. |
|---|---|---|
| Ubiquitinylation kit | 1 Kit (50 rxn) | BML-UW9920-0001 |
