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找出您實驗的最佳轉染試劑

友善列印TransIT-TKO® & TransIT-siQUEST®

  • 適合各種 siRNA / miRNA duplex 轉染實驗使用。
  • 由於不同的細胞株/核酸序列對於轉染試劑的反應皆會有所不同,因此特別設計兩種不同配方的轉染試劑,可供研究者測試找尋最適合自己實驗系統的轉染試劑*。
  • 高運送效率與低細胞毒性,性能超越知名品牌產品。
*不知道何種轉染試劑適合自己的細胞?請將細胞名稱提供給伯森業務專員,我們將協助您取得原廠技術人員專業的建議。

 

產品效能:


Highly Effective Delivery of miRNA Duplexes Using Both the (A) TransIT-TKO® and (B) TransIT-siQUEST® Transfection Reagents. Three miRNA reporter constructs were created in the psiCHECK™-2 Vector (Promega) by cloning the target sequences of miR-18, miR-143, and let7a within the 3' untranslated region of expressed Renilla luciferase mRNA. The psiCHECK™-2 vector also expresses the internal control, firefly luciferase. Each psiCHECK™ reporter plasmid was transfected into both HEK 293 and HeLa cells using the TransIT®-LT1 Reagent. The presence of the specific miRNA is indicated by a decrease in the level of Renilla luciferase activity relative to firefly luciferase activity. Approximately 6 hours post DNA transfection, the cells were transfected with 25 nM miR-18, miR-143 or let7a duplex miRNAs (Ambion) using either the (A) TransIT-TKO® or (B) TransIT-siQUEST® Transfection Reagents. Twenty-four hours post-miRNA transfection, luciferase expression was measured and Renilla luciferase activity was normalized to firefly luciferase activity in each sample. Expression levels were compared to cells transfected in parallel with a negative control miRNA duplex (Ambion).

Greater Knockdown Efficiencies Using TransIT-siQUEST® and TransIT-TKO® Reagents than Lipofectamine™ 2000 (LF2K). Firefly and sea pansy luciferase reporter vectors were co-transfected into various cell lines using the TransIT®-LT1 Reagent. Subsequently, firefly luciferase expression was knocked down by transfection of 25 nM anti-firefly luciferase siRNA using either TransIT-siQUEST® (red), TransIT-TKO® (pink) or LF2K (gray) Reagents. Bars indicate the percent of normalized firefly luciferase expression as compared to each reagent alone control 24 hours post-transfection.

RNA Interference using TransIT-TKO® and TransIT®- siQUEST® Reagent/Anti-Firefly Luciferase siRNA Complexes.
Reporter plasmids expressing firefly and sea pansy luciferase were cotransfected into a variety of cell lines using the appropriate TransIT® plasmid transfection reagent. Firefly luciferase expression was knocked down using anti-firefly luciferase siRNA (25 nM final concentration in well) complexed and delivered with either TransIT-TKO® or TransIT®- siQUEST® Transfection Reagent. Twenty-four hours post-transfection, firefly luciferase expression was determined and normalized to sea pansy luciferase expression in each sample to control for plasmid transfection efficiency. No reduction in sea pansy luciferase expression was observed. The percent decrease of firefly luciferase expression was determined by comparing the normalized firefly luciferase expression to the reagent alone control.

 

訂購資訊

Product Pack Size Number of Transfections in 24-well Plate Cat. No.
TransIT-TKO®
TransIT-TKO® Transfection Reagent 0.4 ml 160 MR-MIR2154
1.5 ml 400 MR-MIR2150
5 x 1.5 ml 2,000 MR-MIR2155
10 x 1.5 ml 4,000 MR-MIR2156
TransIT-siQUEST®
TransIT-siQUEST® Transfection Reagent 0.4 ml 266 MR-MIR2114
1.5 ml 665 MR-MIR2110
5 x 1.5 ml 3,325 MR-MIR2115
10 x 1.5 ml 6,650 MR-MIR2116
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