His-tag 蛋白質純化產品特輯

在使用原核細胞（如大腸桿菌）或真核細胞（如酵母菌、昆蟲細胞或哺乳動物細胞）大量表現重組蛋白質時，通常會在目標蛋白基因的末端銜接上一段特殊可辨識的標籤序列（如 His-tag 或 GST-tag），以便後續藉由親和性層析技術純化取得目標蛋白進行結構或功能分析。

His-tag 標籤通常是由 6~9 個 Histidine 胺基酸所組成，由於 His-tag 標籤短小（常用的 6x-His tag 標籤分子量約僅有 0.8 kDa）不易影響目標蛋白質的摺疊構形，且能夠非常容易地藉由螯合有二價陽離子的層析膠體純化分離出來，因此成為目前最廣為使用的重組蛋白質融合標籤之一。

His-tag fused N or C terminal of the recombinant protein.

常見的 His-tag 蛋白質純化膠體包含鎳離子 (Ni²⁺) 或鈷離子 (Co²⁺) 螯合膠體，一般來說使用鎳離子螯合膠體可以取得較多的純化蛋白質產量，鈷離子螯合膠體則可以提供更佳的純化蛋白質純度。以下就為您整理報導最受科研人員們所青睞的 His-tag 蛋白質純化實驗明星商品。

Ni-NTA Affinity Resin - Amintra

由 Expedeon (part of Abcam) 所開發的 Ni-NTA Affinity Resin - Amintra (ab270549) 能夠自細胞溶解物中快速有效地純化出融合有 His-tag 標籤的重組蛋白，其產品特點包含：

• 高蛋白質結合力 >40 mg 6x His-tagged protein
• 天然 (native) 或變性 (denature) 條件皆可適用
• 純化產物適用於蛋白質結構解析或功能活性分析等實驗

SDS-PAGE and western blot analysis of 8XHis tagged RBD protein of SARS-CoV-2 produced in N. benthamiana. The crude proteins were extracted from plants, 8XHis tagged RBD protein was purified by Ni–NTA affinity resin and analyzed on SDS-PAGE gels and visualized with InstantBlue staining (a). Lane M: protein ladder; Lane 1: total soluble protein of N. benthamiana agroinfiltrated with pBY2e-SARS-CoV-2 RBD; Lane 2: purified SARS-CoV-2 RBD. For western blot analysis, proteins on the blot were probed with rabbit anti-His conjugated with HRP (b). Lane 1: crude extract from non-infiltrated N. benthamiana; Lane 2: total soluble protein of N. benthamiana agroinfiltrated with pBY2e-SARS-CoV-2 RBD; Lane 3: purified SARS-CoV-2 RBD. IMAGE © Sci Rep. 2020 Oct 19;10(1):17698. Figure S1.

產品相關文獻：

• Tit-Oon P, et al. Prediction of the binding interface between monoclonal antibody m102.4 and Nipah attachment glycoprotein using structure-guided alanine scanning and computational docking. Sci Rep. 2020 Oct 26;10(1):18256. PMID: 33106487
• Rattanapisit K, et al. Rapid production of SARS-CoV-2 receptor binding domain (RBD) and spike specific monoclonal antibody CR3022 in Nicotiana benthamiana. Sci Rep. 2020 Oct 19;10(1):17698. PMID: 33077899
• Taylor RL, et al. Loss-of-Function Mutations in the CFH Gene Affecting Alternatively Encoded Factor H-like 1 Protein Cause Dominant Early-Onset Macular Drusen. Ophthalmology. 2019 Oct;126(10):1410-1421. PMID: 30905644

訂購資訊：

相關產品：

BaseMuncher Endonuclease

由 Expedeon (part of Abcam) 所開發的 BaseMuncher Endonuclease (ab270049) 是蛋白質純化實驗的絕佳助手，能夠專一性水解細胞溶解物中的所有 DNA、RNA，降低樣本黏稠度，提升蛋白質純化效率！產品特點包含：

• >99% 高純度核酸水解酶，不具蛋白水解活性
• 活性為 DNAase 的 100 倍
• 超高 C/P 值！具有與 Benzonase 同等的品質與效率，但價格更便宜

DNA digestion with BaseMuncher Endonuclease or nuclease was monitored by agarose gel. 50 mg of salmon sperm DNA was incubated with the indicated units of BaseMuncher Endonuclease (ab270049) or another brand of nuclease at 37°C for 30 mins in a buffer containing 50 mM Tris-HCl; pH 8.0 and 1 mM MgCl₂.

產品相關文獻：

• Rodríguez-Martínez M, et al. Evidence That STK19 Is Not an NRAS-dependent Melanoma Driver. Cell. 2020 Jun 11;181(6):1395-1405.e11. PMID: 32531245
• Jones M, et al. Cryo-EM structures of the XPF-ERCC1 endonuclease reveal how DNA-junction engagement disrupts an auto-inhibited conformation. Nat Commun. 2020 Feb 28;11(1):1120. PMID: 32111838
• Sherlock D, et al. Identification of the First Gene Transfer Agent (GTA) Small Terminase in Rhodobacter capsulatus and Its Role in GTA Production and Packaging of DNA. J Virol. 2019 Nov 13;93(23):e01328-19. PMID: 31534034

訂購資訊：

更多 His-tag 蛋白質實驗產品資訊與最新活動訊息，歡迎洽詢 Abcam 台灣代理 — 伯森生技。您可透過下方連結瀏覽更多相關資訊：

• His-tag protein production and purification
• [產品目錄] Abcam Expedeon 蛋白質體學研究工具
• Western Blot 實驗做不出來？讓 Abcam Checklist 協助你一一排除問題
• Abcam IP 免疫沉澱實驗產品特輯