Abcam 細胞螢光染劑 | 細胞胞器-細胞結構-活細胞染色 • 粒線體膜電位分析 • 鈣離子流動檢測 • 神經科學影像探針

Abcam 細胞螢光染劑

細胞胞器-細胞結構-活細胞染色
粒線體膜電位分析
鈣離子流動檢測
神經科學影像探針
封片膠 (Mounting Medium)

 

細胞胞器-細胞結構-活細胞染色

Abcam 細胞螢光染劑系列產品能夠讓您免除使用抗體,藉由簡易的染色步驟就能精確地檢測各種細胞胞器 (cell organelles) 與細胞構造,例如:內質網 (endoplasmic reticulum, ER)、高基氏體 (Golgi apparatus)、溶酶體 (lysosome)、粒線體 (mitochondria)、纖維形肌動蛋白 (filamentous actin, F-actin)、細胞膜 (cell memrbane)、細胞核 (cell nucleus)、核仁 (nucleolus)、DNA 等。Abcam 細胞螢光染劑系列產品具有高效穩定的產品效能,以及多種螢光標定選擇,適用於活細胞影像觀察 (live cell imaging)、細胞存活率 (cell viability)、細胞增生 (cell proliferation)、細胞血統追蹤 (cell lineage tracing) 等多種分析實驗。

ER Staining Kit - Red Fluorescence - Cytopainter (ab139482) for endoplasmic reticulum staining

ER Staining Kit - Red Fluorescence - Cytopainter (ab139482) for endoplasmic reticulum staining. Live HeLa cells stained with Red Detection Reagent (A), Hoechst dye (B) and resulting composite image (C). The ER staining kit (ab139482) is specifically designed for use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFPs, CFPs, YFPs). Additionally, a nuclear counterstain (Hoechst 33342) is also included in the kit.

F-actin Staining Kit - Blue Fluorescence - Cytopainter (ab112124) for F-actins staining

F-actin Staining Kit - Blue Fluorescence - Cytopainter (ab112124) for actin filaments staining. Embedded mouse embryonic stem cell-differentiated embryoid bodies (EBs) frozen sections are stained with Rabbit anti laminin alpha 1, Goat Anti-Rabbit IgG H&L (Cy5®) preadsorbed secondary antibody (ab97077) and F-actin Staining Kit - Blue Fluorescence - Cytopainter (ab112124). Image key: red (laminin alpha 1), blue (F-actin), green (nuclei SYTO 16).

Organelle and Cell Structure Detection Dyes
Detect Target Product Name Ex/Em (nm) Sample Cat. No.
ER ER Staining Kit - Green Fluorescence - Cytopainter 441/551 Live/Fixed cells ab139481
ER Staining Kit - Red Fluorescence - Cytopainter 580/677 Live/Fixed cells ab139482
Golgi Golgi Staining Kit - Green Fluorescence - Cytopainter 473/534 Live/Fixed cells ab139483
Lysosome Lysosomal Staining Kit - Blue Fluorescence - Cytopainter 350/440 Live cells ab112135
Lysosomal Staining Reagent - Blue - Cytopainter 353/442 Live cells ab176825
Lysosomal Staining Kit - Green Fluorescence (Ex405nm) - Cytopainter 405/480 Live cells ab112138
Lysosomal Staining Reagent - Green - Cytopainter 450/505 Live cells ab176826
Lysosomal Staining Kit - Green Fluorescence - Cytopainter 490/525 Live cells ab112136
Lysosomal Staining Reagent - Orange - Cytopainter 542/556 Live cells ab176827
Lysosomal Staining Kit - Orange - Cytopainter 540/560 Live cells ab138895
Lysosomal Staining Reagent - Red - Cytopainter 575/597 Live cells ab176828
Lysosomal Staining Kit - Red Fluorescence - Cytopainter 575/600 Live cells ab112137
Lysosomal Staining Reagent - Deep Red - Cytopainter 596/619 Live cells ab176829
Lysosomal Staining Kit - Deep Red - Cytopainter 590/620 Live cells ab138896
Lysosomal Staining Kit - NIR Fluorescence - Cytopainter 630/650 Live cells ab176746
Lysosomal Staining Reagent - NIR - Cytopainter 636/650 Live cells ab176824
Golgi / ER Golgi/ER Staining Kit - Cytopainter 473/534 (Golgi)
580/677 (ER)
Live cells ab139485
Lysosome / ER / Nuclear ER/Lysosome/Nuclear Staining Reagent 481/544 (Lysosome)
580/677 (ER)
350/461 (Nuclear)
Live cells ab139489
Lysosome/ER/Nuclear Staining Reagent - Cytopainter 440/565 (ER)
568/667 (Lysosome)
350/461 (Nuclear)
Live cells ab139488
Mitochondria Mitochondrial Staining Kit - Blue - Cytopainter 360/445 Live cells ab219939
Mitochondrial Staining Reagent - Blue - Cytopainter 350/490 Live cells ab219940
Mitochondrial Staining Kit - Green Fluorescence - Cytopainter 490/520 Live cells ab112143
Mitochondrial Staining Reagent - Green - Cytopainter 498/520 Live cells ab176830
Mitochondrial Staining Kit - Orange (Ex405nm) - Cytopainter 405/550 Live cells ab138897
Mitochondrial Staining Reagent - Orange 405 - CytoPainter 399/550 Live cells ab176834
Mitochondrial Staining Kit - Orange Fluorescence - Cytopainter 545/575 Live cells ab112144
Mitochondrial Staining Reagent - Orange - Cytopainter 545/575 Live cells ab176831
Mitochondrial Staining Kit - Red Fluorescence - Cytopainter 580/600 Live cells ab112145
Mitochondrial Staining Reagent - Red - Cytopainter 575/600 Live cells ab176832
Mitochondrial Staining Reagent - NIR - Cytopainter 640/659 Live cells ab176833
Mitochondrial Staining Kit - NIR Fluorescence - Cytopainter 640/660 Live cells ab176747
F-actin F-actin Staining Kit - Blue Fluorescence - Cytopainter 350/450 Fixed cells ab112124
F-actin Staining Kit - Green Fluorescence - Cytopainter 500/520 Fixed cells ab112125
F-actin Staining Kit - Orange Fluorescence - Cytopainter 550/575 Fixed cells ab112126
F-actin Staining Kit - Red Fluorescence - Cytopainter 594/610 Fixed cells ab112127
Nucleolus Nucleolar Staining Kit - Green Fluorescence - Cytopainter 450/481 Live cells ab139475
Nucleolus / Nucleus Nucleolar/Nuclear Staining Kit - Green/ Red Fluorescence - Cytopainter 450/490 (Nucleolus)
566/650 (Nucleus)
Live cells ab139474
Cell Membrane Cell Plasma Membrane Staining Kit - Orange Fluorescence - Cytopainter 540/590 Live cells ab219941
Cell Plasma Membrane Staining Kit - Deep Red Fluorescence - Cytopainter 640/660 Live cells ab219942

Immunocytochemistry/ Immunofluorescence - DRAQ5™ (ab108410)

Immunocytochemistry/ Immunofluorescence - DRAQ5™ (ab108410). HeLa cells were stained with Anti-beta Catenin antibody (ab16051) and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291). The secondary antibodies were Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899) and Goat Anti-Mouse IgG H&L (DyLight® 594) preadsorbed (ab96881). DRAQ5™ (ab108410) was added to the secondary antibody mixture to label nuclear DNA (pseudocolor orange). Image key: green (beta Catenin), red (alpha Tubulin), orange (nuclei DRAQ5™).

DNA Detection Dyes
Product Name Ex/Em (nm) Sample Cat. No.
DAPI Staining Solution 358/461 (DNA)
358/500 (RNA)
Live/Fixed cells ab228549
Hoechst 33258 Staining Dye Solution 352/461 Live/Fixed cells ab228550
Hoechst 33342 Staining Dye Solution 350/461 Live/Fixed cells ab228551
Nuclear yellow (Hoechst S769121) 355/495 Live/Fixed cells ab138903
Propidium Iodide 536/617 Fixed cells ab14083
DRAQ5™ 646/697 Live/Fixed cells ab108410
DRAQ7™ 599/697 Fixed cells ab109202

 

Cell Viability Assay Kits (Fluorescent)
Product Name Ex/Em (nm) Cat. No.
Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) 405/460 ab176748
Cell Viability Assay Kit (Fluorometric - Blue) 350/461 ab112120
Cell Viability Assay Kit (Fluorometric - Green) 490/520 ab112122
Cell Viability Assay Kit (Fluorometric - Dual Green/Red) 490/525 (Live cells)
540/620 (Dead cells)
ab112121
Live and Dead Cell Assay 494/515 (Live cells)
528/617 (Dead cells)
ab115347
Fixable Cell Viability Assay Kit (Fluorometric - Blue) - Cytopainter 353/442 ab176741
Fixable Cell Viability Assay Kit (Fluorometric - Blue Ex 405 nm) - Cytopainter 410/450 ab176738
Fixable Cell Viability Assay Kit (Fluorometric - Green Ex 405 nm) - CytoPainter 408/512 ab176739
Fixable Cell Viability Assay Kit (Fluorometric - Green) - Cytopainter 498/521 ab176742
Fixable Cell Viability Assay Kit (Fluorometric - Orange Ex 405 nm) - Cytopainter 398/550 ab176740
Fixable Cell Viability Assay Kit (Fluorometric - Orange) - Cytopainter 547/573 ab176743
Fixable Cell Viability Assay Kit (Fluorometric - Red) - CytoPainter 583/603 ab176744
Fixable Cell Viability Assay Kit (Fluorometric - Deep Red) - Cytopainter 649/660 ab176745

Immunocytochemistry/ Immunofluorescence - EdU Assay / EdU Staining Proliferation Kit (iFluor 488) (ab219801)

Immunocytochemistry/ Immunofluorescence - EdU Assay / EdU Staining Proliferation Kit (iFluor 488) (ab219801). EdU Staining Proliferation Kit (iFluor 488) (ab219801) staining of proliferating cells. HeLa (Human epithelial cell line from cervix adenocarcinoma) cells (4 x 10⁴ cells/well in 96 plate) were incubated with 10 μM EdU for 3 hours. Cells were analyzed using a TCS SP8 confocal microscope (Leica-Microsystems). DNA (blue) was staining with Hoechst 33342 (ab145597). Green cells show EdU/Hoechst-positive cells.

Cell Proliferation Assay and Cell Cycle Assay Kits (Fluorescent)
Assay Type Product Name Ex/Em (nm) Cat. No.
Nucleoside analogs EdU Assay / EdU Staining Proliferation Kit (iFluor 488) 491/520 ab219801
EdU Assay / EdU Staining Proliferation Kit (iFluor 647) 649/664 ab222421
DNA-staining dyes Cell Cycle Assay Kit (Fluorometric - Green) 490/520 ab112116
Cell Cycle Assay Kit (Fluorometric - Red) 490/620 ab112117
Propidium Iodide Flow Cytometry Kit 536/617 ab139418
Dye dilution assays CFSE - Cell Labeling Kit 492/517 ab113853
Cell Proliferation Staining Reagent - Blue Fluorescence (Ex 405 nm) - Cytopainter 403/454 ab176726
Cell Proliferation Staining Reagent - Green Fluorescence - Cytopainter 511/525 ab176735
Cell Proliferation Staining Reagent - Orange Fluorescence - Cytopainter 542/556 ab176737
Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter 628/643 ab176736

 

Cell Tracking Kits (Fluorescent)
Product Name Ex/Em (nm) Cat. No. Note
Cell Tracking Dye Kit - Blue - Cytopainter 360/445 ab138890 Cells need to be viewed in 24 - 48 hours as the dye will not pass to daughter cells
Cell Tracking Dye Kit - Green - Cytopainter 490/520 ab138891
Cell Tracking Dye Kit - Orange - Cytopainter 540/560 ab138892
Cell Tracking Dye Kit - Red - Cytopainter 575/600 ab138893
Cell Tracking Dye Kit - Deep Red - Cytopainter 630/650 ab138894
Cell Tracking Red Dye Kit - Longer cell staining, DMSO-free 554/575 ab269446 Enable longer cell staining (up to 20 days) without toxicity

 

粒線體膜電位分析

粒線體膜電位 (mitochondrial membrane potential) 下降是細胞凋亡 (apoptosis) 的指標之一,同時也是調控細胞凋亡的重要關鍵。已有多項研究證實,癌細胞往往具有異常高的粒線體膜電位,透過降低癌細胞粒線體膜電位,可促使癌細胞走向細胞凋亡 [1]。Abcam 提供多款活細胞分析用螢光染劑產品,可協助您精確檢測藥物處理後的細胞粒線體膜電位變化。

Mitochondrial membrane potential was measured using TMRE-Mitochondrial Membrane Potential Assay Kit (ab113852)

Mitochondrial membrane potential was measured using TMRE-Mitochondrial Membrane Potential Assay Kit (ab113852). P19 neurons (750 cells/mm²) were exposed to MDMA on days 7–9 in serum-free medium for 10 min up to 48 hours. The positive control FCCP (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone), an uncoupler of mitochondrial oxidative phosphorylation, was applied at the concentration of 5 μM for 10 min. The cells were incubated with 500 μM TMRE for 30–45 min at 37°C, 5% CO₂, followed by washing once with 100 μl of HBSS containing 0.2% bovine serum albumin. A volume of 200 μl of HBSS containing 0.2% bovine serum albumin was added to each well, and the fluorescence was measured with excitation/emission: 544/590 nm. IMAGE © PLoS One. 2016 Nov 18;11(11):e0166750. Fig 5 [2].

Mitochondrial Membrane Potential Assay Kits (Fluorescent)
Type Product Name Ex/Em (nm) Cat. No.
JC-1 JC-1 - Mitochondrial Membrane Potential Assay Kit 475/530
475, 535/590
Green is increased in apoptotic cells
ab113850
JC-10 JC-10 Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) 490/520
490/570
Green is increased in apoptotic cells
ab112133
JC-10 Mitochondrial Membrane Potential Assay Kit (Microplate) 490/520
490/570
Green is increased in apoptotic cells
ab112134
MitoOrange dye Orange Mitochondrial Membrane Potential Assay Kit (Flow Cytometry) 540/590
Fluorescence intensity is decreased in apoptotic cells
ab138898
Orange Mitochondrial Membrane Potential Assay Kit (Microplate) 540/590
Fluorescence intensity is decreased in apoptotic cells
ab138899
MitoNIR dye NIR Mitochondrial Membrane Potential Assay Kit (Microplate) 640/680
Fluorescence intensity is decreased in apoptotic cells
ab112150
TMRE TMRE-Mitochondrial Membrane Potential Assay Kit 549/575
Fluorescence intensity is decreased in apoptotic cells
ab113852
TMRM TMRM Assay Kit (Mitochondrial Membrane Potential) 548/573
Fluorescence intensity is decreased in apoptotic cells
ab228569

 

鈣離子流動檢測

鈣離子 (calcium ions, Ca²⁺) 是重要的第二傳訊者 (second messenger),參與調節多項細胞機轉,包含細胞增生、基因轉錄、肌肉收縮、內吞作用 (endocytosis) 等。無論是 G 蛋白偶聯受體 (G protein-coupled receptors, GPCRs) 藥物開發或是神經元 (neuron) 功能研究,都能透過監測細胞內鈣離子在特定時間與空間條件下的濃度變化,深入了解在藥物作用或特定生理狀態下細胞內的訊息傳遞路徑。Abcam 提供多款螢光染劑產品,可協助您精確檢測活細胞內鈣離子濃度的變化。

Functional Studies - Fluo-8 No Wash Calcium Assay kit (ab112129)

Fluo-8 vs Fluo-4 sensitivity to calcium release in HEK-293 cells induced by Carbachol. Carbachol Dose Response was measured in HEK293 cells with Fluo-8 Calcium Flux Assay Kit - No Wash (ab112129) and a Fluo-4 calcium assay kit. HEK293 cells were seeded overnight at 40,000 cells/100 µl/well in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µl of dye-loading solution using the ab112129 or the Fluo-4 kit for 1 hour at room temperature. Carbachol (50 µl/well) was added to achieve the final concentrations indicated.

ATP Dose Response in CHO cells measured with Fura-2 Calcium Flux Assay Kit - No Wash, Ratiometric (ab176766)

ATP Dose Response in CHO cells measured with Fura-2 Calcium Flux Assay Kit - No Wash, Ratiometric (ab176766). CHO-K1 cells were seeded overnight at 40,000 cells/100 μl/well in a Costar black wall/clear bottom 96-well plate. The cells were incubated with 100 μl of the Fura-2 Calcium Flux Assay Kit - No Wash, Ratiometric (ab176766) for 1 hour at room temperature. ATP (50 μl/well) was added to achieve the final indicated concentrations.

Calcium Indicators and Assay Kits (Fluorescent)
Type Product Name Ex/Em (nm) Cat. No.
Calbryte 520 AM Calcium Flux Assay Kit (Flow cytometry) 490/525 ab233472
Fluo Fluo-2 AM, green fluorescent Ca²⁺ binding dye 490/520 ab142775
Fluo-3 AM, Fluorescent calcium Indicator 506/526 ab145254
Fluo-4 AM, Fluorescent labeling reagent 494/516 ab241082
Fluo-4 Assay Kit (Calcium) 490/525 ab228555
Fluo-5 AM, Fluorescent labeling reagent (Fluo-4 analogue) 494/516 ab241083
Fluo-8 AM, green fluorescent calcium binding dye 490/520 ab142773
Fluo-8 Calcium Assay Kit - Medium Removal 490/525 ab112128
Fluo-8 Calcium Flux Assay Kit - No Wash 490/525 ab112129
Fura Fura-2 AM, Ca²⁺ selective fluorescent indicator 340, 380/505 ab120873
Fura-2 Calcium Flux Assay Kit - No Wash, Ratiometric 340, 380/510 ab176766
Indo Indo-1 AM, fluorescent Ca²⁺ indicator 346/475, 405 ab142778
Rhod Rhod-2 AM, fluorescent Ca²⁺ indicator 552/581 ab142780
Rhod-4 Calcium Assay kit - Medium Removal No Wash 540/590 ab112156
Rhod-4 Calcium Assay Kit - No Wash 540/590 ab112157

 

神經科學影像探針

Abcam 阿茲海默症 (Alzheimer's disease, AD) 研究用近紅外光螢光探針 (near infrared fluorescent probes, NIRF probes) 系列產品可穿透血腦障壁 (blood brain barrier, BBB),專一性檢測沈積於腦部的乙型類澱粉 (β-amyloid, Aβ)。具有高專一性、高訊噪比、安全、經濟等優質特性,特別適合應用於活體動物即時影像觀察實驗 (in vivo real-time imaging)。

Abcam NIRF probes for real-time imaging of amyloid beta

NIRF Probes for Real-time Imaging of Amyloid Beta
Detect Target Product Name Ex/Em (nm) Cat. No.
Aβ42 fibrils MCAAD-3, Near-infrared probe for imaging of amyloid plaques 596/685 ab216983
Aβ40 fibrils CRANAD-2, Near-infrared Amyloid-beta fluorescent probe 640/715 ab141775

 

封片膠 (Mounting Medium)

Abcam 封片膠產品可以有效減緩螢光分子氧化褪色的情形,提供良好持久的保護效果。

Antifade Mounting Medium
Product Name Counterstain Cat. No.
Mounting Medium With DAPI - Aqueous, Fluoroshields DAPI ab104139
Mounting Medium With PI - Aqueous, Fluoroshield PI ab104129
Anti-Fade Fluorescence Mounting Medium - Aqueous, Fluoroshield ab104135
Glycerol Mounting Medium - Anti-Fade with DAPI DAPI ab188804

完整產品資訊與最新活動訊息,歡迎洽詢 Abcam 台灣代理 — 伯森生技。有任何免疫細胞化學染色 (​immunocytochemistry, ICC)、免疫螢光染色 (Immunofluorescence, IF) 實驗問題或疑難雜症,都歡迎隨時找我們交流討論喔~ (👨‍💻伯森生技線上線下聯絡資訊

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References

  1. Jeena MT, Kim S, Jin S, Ryu JH. Recent Progress in Mitochondria-Targeted Drug and Drug-Free Agents for Cancer Therapy. Cancers (Basel). 2019 Dec 18;12(1):4. PMID: 31861339
  2. Popova D, Forsblad A, Hashemian S, Jacobsson SO. Non-Serotonergic Neurotoxicity by MDMA (Ecstasy) in Neurons Derived from Mouse P19 Embryonal Carcinoma Cells. PLoS One. 2016 Nov 18;11(11):e0166750. PMID: 27861613
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