染色質重組分析試劑

在真核細胞核內，Genomic DNA 會包裹纏繞著 Histone 蛋白形成核小體 (nucleosome)，並進一步形成複雜的的立體結構 — 染色質 (chromatin)。藉由 Genomic DNA 的甲基化程度以及 Histone 蛋白的後轉譯修飾類型，可影響染色質的纏繞鬆緊度，進而調控基因的表現與否。一般來說 Promoter 區域的低甲基化 (hypomethylation) 以及 Histone 蛋白的乙醯化 (acetylation) ，皆有助於轉錄因子與 Genomic DNA 結合促使基因表現。

EMSA 以外的優質新選擇：
在進行 DNA-Protein interactions 實驗時，通常會選用 EMSA 作為分析方法。而透過 Alpha 技術所建立的分析平台，除了可進行同樣的實驗偵測以外，還能讓研究者免除使用放射性探針以及跑膠的流程，並提供更寬廣的偵測區間 (dynamic range)、以及精確的定量結果。下圖即是芬蘭土庫大學 Vuori 博士發表於 FEBS 期刊中 EMSA 與 Alpha 技術的比較圖。

Photo Source: FEBS J. 2009 Dec;276(24):7366-74.

	EMSA	Alpha
Probe type	³²P-labeled	Biotin-labeled
Gel electrophoresis	Yes	No
Time to read	>18 hours	~2 hours
Analytical range	Narrow	Broad
Result	Semi-quantitative	Precise quantitative
Throughput	Low	High

High Sensitivity and Superior Signal to Background Ratio! 以 Alpha 技術偵測 Sp1 transcription factor 與相對應 DNA 序列的結合情形。結果顯示僅需使用 1 µg/well 的 Hep G2 nuclear extracts，以及 10 nM 的 Biotin 標定探針，即可得到高達 50.4 的訊噪比 (signal to background)。

Alpha Toolbox & AlphaScreen Fusion Detection Kit
Alpha Toolbox 與 AlphaScreen Fusion Detection Kit 系列產品，可以讓研究者自由組合出多種偵測平台，以彈性符合多元化的實驗需求與生物樣本特性。(點擊下表品項，即可開啟詳細產品說明)

Alpha Toolbox

AlphaScreen Fusion Tag
Detection Kit¹

Alpha Donor Bead

Anti-FLAG
Anti-Mouse IgG
Anti-Rabbit IgG
Glutathione(GSH)
Nickel Chelate
Protein A
Strep-Tactin
Streptavidin
Unconjugated

AlphaLISA Accetor Bead

Anti-6xHis
Anti-Chicken IgY
Anti-c-Myc
Anti-DIG
Anti-FITC
Anti-FLAG
Anti-GFP
Anti-Goat IgG
Anti-GST
Anti-Human IgG
Anti-MBP
Anti-Mouse IgG
Anti-Mouse IgM

Anti-Rabbit IgG
Anti-Rat IgG
Anti-Sheep IgG
Anti-V5
Glutathione(GSH)
Nickel Chelate
Protein A
Protein G
Protein L
Strep-Tactin
Streptavidin
Unconjugated

c-Myc
DIG
FITC
FLAG
GST
HA
Histidine (Nickel Chelate)
Biotinylated-GST²
Biotinylated-HIS²

(註1)	Kit 中皆含有 AlphaScreen Streptavidin coated Donor Bead 與 AlphaScreen anti-tag antibody coated Acceptor Bead。
(註2)	可作為 Alpha 實驗中的 Positive control 使用。

AlphaScreen Acceptor Bead

Protein A
Unconjugated

訂購資訊 (請點擊下列分類選項，瀏覽詳細訂購資訊)

Alpha Donor Bead

Product	Pack Size	Cat. No.
Anti-FLAG Alpha Donor Beads	1 mg	PK-AS103D
	5 mg	PK-AS103M
	25 mg	PK-AS103R
Anti-Mouse IgG Alpha Donor Beads	1 mg	PK-AS104D
	5 mg	PK-AS104M
	25 mg	PK-AS104R
Anti-Rabbit IgG Alpha Donor Beads	1 mg	PK-AS105D
	5 mg	PK-AS105M
	25 mg	PK-AS105R
AlphaScreen Glutathione Donor Beads	1 mg	PK-6765300
	5 mg	PK-6765301
	25 mg	PK-6765302
AlphaScreen Nickel Chelate Donor Beads	1 mg	PK-AS101D
	5 mg	PK-AS101M
	25 mg	PK-AS101R
Protein A Alpha Donor Beads	1 mg	PK-AS102D
	5 mg	PK-AS102M
	25 mg	PK-AS102R
Strep-Tactin Alpha Donor Beads	1 mg	PK-AS106D
	5 mg	PK-AS106M
	25 mg	PK-AS106R
AlphaScreen Streptavidin Donor Beads	1 mg	PK-6760002S
	5 mg	PK-6760002
	50 mg	PK-6760002B
AlphaScreen Unconjugated Donor Beads	1 mg	PK-6762013
	5 mg	PK-6762011
	50 mg	PK-6762012

AlphaLISA Acceptor Bead

Product	Pack Size	Cat. No.
Anti-6xHis AlphaLISA Acceptor Beads	250 µg	PK-AL128C
	5 mg	PK-AL128M
	50 mg	PK-AL128R
Anti-Chicken IgY AlphaLISA Acceptor Beads	250 µg	PK-AL131C
	5 mg	PK-AL131M
	50 mg	PK-AL131R
Anti-c-Myc AlphaLISA Acceptor Beads	250 µg	PK-AL111C
	5 mg	PK-AL111M
	50 mg	PK-AL111R
Anti-DIG AlphaLISA Acceptor Beads	250 µg	PK-AL113C
	5 mg	PK-AL113M
	50 mg	PK-AL113R
Anti-FITC AlphaLISA Acceptor Beads	250 µg	PK-AL127C
	5 mg	PK-AL127M
	50 mg	PK-AL127R
Anti-FLAG AlphaLISA Acceptor Beads	250 µg	PK-AL112C
	5 mg	PK-AL112M
	50 mg	PK-AL112R
Anti-GFP AlphaLISA Acceptor Beads	250 µg	PK-AL133C
	5 mg	PK-AL133M
	50 mg	PK-AL133R
Anti-Goat IgG (Fc specific) AlphaLISA Acceptor Beads	250 µg	PK-AL107C
	5 mg	PK-AL107M
	50 mg	PK-AL107R
Anti-GST AlphaLISA Acceptor Beads	250 µg	PK-AL110C
	5 mg	PK-AL110M
	50 mg	PK-AL110R
Anti-Human IgG (Fc-specific) AlphaLISA Acceptor Beads	250 µg	PK-AL103C
	5 mg	PK-AL103M
	50 mg	PK-AL103R
Anti-Maltose-Binding Protein (MBP) AlphaLISA Acceptor Beads	250 µg	PK-AL134C
	5 mg	PK-AL134M
	50 mg	PK-AL134R
Anti-Mouse IgG (Fc-specific) AlphaLISA Acceptor Beads	250 µg	PK-AL105C
	5 mg	PK-AL105M
	50 mg	PK-AL105R
Anti-Mouse IgM AlphaLISA Acceptor Beads	250 µg	PK-AL130C
	5 mg	PK-AL130M
	50 mg	PK-AL111R
Anti-Rabbit IgG (Fc-specific) AlphaLISA Acceptor Beads	250 µg	PK-AL104C
	5 mg	PK-AL104M
	50 mg	PK-AL104R
Anti-Rat IgG (Fc-specific) AlphaLISA Acceptor Beads	250 µg	PK-AL106C
	5 mg	PK-AL106M
	50 mg	PK-AL106R
Anti-Sheep IgG (Fc specific) AlphaLISA Acceptor Beads	250 µg	PK-AL132C
	5 mg	PK-AL132M
	50 mg	PK-AL132R
Anti-V5 AlphaLISA Acceptor Beads	250 µg	PK-AL129C
	5 mg	PK-AL129M
	50 mg	PK-AL129R
Glutathione AlphaLISA Acceptor Beads	250 µg	PK-AL109C
	5 mg	PK-AL109M
	50 mg	PK-AL109R
Nickel Chelate AlphaLISA Acceptor Beads	250 µg	PK-AL108C
	5 mg	PK-AL108M
	50 mg	PK-AL108R
Protein A AlphaLISA Acceptor Beads	250 µg	PK-AL101C
	5 mg	PK-AL101M
	50 mg	PK-AL101R
Protein G AlphaLISA Acceptor Beads	250 µg	PK-AL102C
	5 mg	PK-AL102M
	50 mg	PK-AL102R
Protein L AlphaLISA Acceptor Beads	250 µg	PK-AL126C
	5 mg	PK-AL126M
	50 mg	PK-AL126R
Strep-Tactin AlphaLISA Acceptor Beads	250 µg	PK-AL136C
	5 mg	PK-AL136M
	50 mg	PK-AL136R
Streptavidin AlphaLISA Acceptor Beads	250 µg	PK-AL125C
	5 mg	PK-AL125M
	50 mg	PK-AL125R
Unconjugated AlphaLISA Acceptor Beads	1 mg	PK-6772001
	5 mg	PK-6772002
	50 mg	PK-6772003

AlphaScreen Acceptor Bead

AlphaScreen Fusion Tag Detection Kit

Product	Pack Size	Cat. No.
AlphaScreen c-Myc Detection Kit	500 assay	PK-6760611C
	10,00 assay	PK-6760611M
	50,00 assay	PK-6760611R
AlphaScreen DIG (Digoxin/Digoxigenin) Detection Kit	500 assay	PK-6760604C
	10,00 assay	PK-6760604M
	50,00 assay	PK-6760604R
AlphaScreen FITC (Fluorescein) Detection Kit	500 assay	PK-6760605C
	10,00 assay	PK-6760605M
	50,00 assay	PK-6760605R
AlphaScreen FLAG Detection Kit	500 assay	PK-6760613C
	10,00 assay	PK-6760613M
	50,00 assay	PK-6760613R
AlphaScreen GST Detection Kit	500 assay	PK-6760603C
	10,00 assay	PK-6760603M
	50,00 assay	PK-6760603R
AlphaScreen HA (Hemagglutinin) Detection Kit	500 assay	PK-6760612C
	10,00 assay	PK-6760612M
	50,00 assay	PK-6760612R
AlphaScreen Histidine (Nickel Chelate) Detection Kit	500 assay	PK-6760619C
	10,00 assay	PK-6760619M
	50,00 assay	PK-6760619R
AlphaScreen Biotinylated-GST	10,00 assay	PK-6760305M
AlphaScreen Biotinylated-HIS	10,00 assay	PK-6760303M

Other Alpha Products

Classification	Product	Pack Size	Cat. No.
AlphaLISA Buffers	AlphaLISA Dissociation Buffer, 3X	10 ml	PK-AL006C
	AlphaLISA Dissociation Buffer, 3X	100 ml	PK-AL006F
	AlphaLISA HiBlock Buffer, 10X	10 ml	PK-AL004C
	AlphaLISA HiBlock Buffer, 10X	100 ml	PK-AL004F
	AlphaLISA Immunoassay Buffer, 10X	10 ml	PK-AL000C
	AlphaLISA Immunoassay Buffer, 10X	100 ml	PK-AL000F
	AlphaLISA Lysis Buffer, 5X	10 ml	PK-AL003C
	AlphaLISA Lysis Buffer, 5X	100 ml	PK-AL003F
	AlphaLISA NaCl Buffer, 5X	10 ml	PK-AL007C
	AlphaLISA NaCl Buffer, 5X	100 ml	PK-AL007F
	AlphaLISA Universal Buffer, 5X	10 ml	PK-AL001C
	AlphaLISA Universal Buffer, 5X	100 ml	PK-AL001F
Bead Conjugation Kit	AlphaScreen Conjugation Kit Each Kit Contains: Streptavidin Alpha Donor Bead, unconjugated AlphaScreen Acceptor Bead.	2 x 2 mg	PK-6760000K
Optimization Tools	AlphaScreen Omnibeads OmniBead are designed as a tool to troubleshoot signal variability. The Bead contain all of the chemical components needed to generate a strong AlphaScreen signal without the presence of AlphaScreen Donor or Acceptor Bead.	1,000 assay	PK-6760626D
		10,00 assay	PK-6760626M
		50,00 assay	PK-6760626R
	AlphaScreen TruHits Kit Each Kit Contains: Biotinylated Acceptor Bead, streptavidin Alpha Donor Bead for identifying false positives.	1,000 assay	PK-6760627D
		10,00 assay	PK-6760627M