LANCE Ultra Kinase Assay Reagents

LANCE Ultra 是由美商 Revvity 公司所研發出的新型態試管內激酶活性分析 (In Vitro Kinase Assay) 技術。此技術幾乎不具有任何技術門檻，只要會使用 Pipetman 就可以進行實驗！流程簡單，從拿到待測檢體開始到取得偵測結果，只要三個步驟就能完成。LANCE Ultra 除了具有無技術門檻以及操作流程簡單的特性之外，還擁有優異的偵測靈敏度與偵測區間，不但可減少樣本用量、提高解析度、並且具有量化的數據顯示方式，非常適合應用於激酶活性分析或篩選激酶活性調控藥物。

產品特點：

• 實際經過 >310 種不同廠商來源市售 Kinases 產品實驗測試 (225 種 Ser/Thr Kinases、85 種 Tyrsoine Kinases)，可提供詳盡的測試條件與 S/B 比值資訊供研究者參考。
• 全均質化 (homogeneous) 實驗流程，流程簡單且不需分離或洗滌步驟，可快速完成偵測實驗。
• 偵測效能穩定，平均 Z' 標準值大於 0.8，確保實驗數據可信度。

作用原理：

LANCE Ultra 是一種時差性螢光共振能量轉移偵測平台 (Time-Resolved Fluoresence Resonance EnergyTransfer assay, TR-FRET assay)，其作用原理是藉由時差性螢光分子 "Eu Chelate" 作為能量捐贈者 (energy donor)，在一定的作用距離下 (約 10 nm) 可將能量轉移至鄰近的 "ULight" 螢光分子激發其產生特定波長的螢光訊號供實驗者偵測。

以 Kinase assay 為例，當 Kinase 將標定有 ULight 螢光分子的 peptide substrate 磷酸化後，帶有 Eu 螢光分子的專一性磷酸化抗體即可結合於 peptide substrate 上，使得 Eu Chelate (能量捐贈者) 與 ULight (能量接受者) 兩者相近。此時以 320 nm 或 340 nm 雷射光激發，即可藉由能量轉移使 ULight 螢光分子發射出 665 nm 的螢光訊號。研究者可藉由偵測此螢光訊號的強弱，應用於 Kinase Inhibitor 藥物開發等…相關研究。

訂購資訊 (請點擊下列分類選項，瀏覽詳細訂購資訊)

Eu-labeled Antibody

Product Pack Size Cat. No. LANCE Eu-W1024 phospho-(Ser) 14-3-3 binding motif antibody 10 μg PK-AD0190 LANCE Eu-W1024 phospho-(Ser) 14-3-3 binding motif 4E2 antibody 10 μg PK-AD0192 LANCE Ultra Europium-anti-phospho-40S Ribosomal Protein S6 (Ser235/236) 10 μg PK-TRF0217-D 100 μg PK-TRF0217-M LANCE Ultra Europium-anti-phospho-Acetyl-CoA Carboxylase (Ser79) Antibody 10 μg PK-TRF0208-D 100 μg PK-TRF0208-M LANCE Eu-W1024 phospho-Akt substrate antibody 10 μg PK-AD0184 LANCE Ultra Europium-anti-phospho-ATF-2 (Thr53) Antibody 10 μg PK-TRF0212-D 100 μg PK-TRF0212-M LANCE Ultra Europium-anti-phospho-CREB (Ser133) 10 μg PK-TRF0200-D 100 μg PK-TRF0200-M LANCE Ultra Europium-anti-phospho-DNA Topoisomerase 2-alpha (Thr1342) 10 μg PK-TRF0218-D 100 μg PK-TRF0218-M LANCE Ultra Europium-anti-phospho-eIF4E-binding protein 1 (Thr37/46) 10 μg PK-TRF0216-D 100 μg PK-TRF0216-M LANCE Ultra Europium-anti-phospho-Glycogen Synthase (Ser641) 10 μg PK-TRF0220-D 100 μg PK-TRF0220-M LANCE Ultra Europium-anti-phospho-Crosstide (GSK-3α Ser21) 10 μg PK-TRF0202-D 100 μg PK-TRF0202-M LANCE Ultra Europium-anti-phospho-Histone H3 (Ser10) Antibody 10 μg PK-TRF0210-D 100 μg PK-TRF0210-M LANCE Ultra Europium-anti-phospho-Histone H3 (Thr3) Antibody 10 μg PK-TRF0211-D 100 μg PK-TRF0211-M LANCE Ultra Europium-anti-phospho-IkappaB-alpha (Ser32/36) 10 μg PK-TRF0206-D 100 μg PK-TRF0206-M LANCE Ultra Europium-anti-phospho-MAP Kinase p38-alpha (Thr180/Tyr182) 10 μg PK-TRF0219-D 100 μg PK-TRF0219-M LANCE Ultra Europium-anti-phospho-mTOR (Ser2448) Antibody 10 μg PK-TRF0209-D 100 μg PK-TRF0209-M LANCE Ultra Europium-anti-phospho-MEK1/2 (Ser217/221) Antibody 10 μg PK-TRF0213-D 100 μg PK-TRF0213-M LANCE Ultra Europium-anti-phospho-Myelin Basic Protein (MBP) 10 μg PK-TRF0201-D 100 μg PK-TRF0201-M LANCE Ultra Europium-anti-phospho-p70 S6K (Thr389) Antibody 10 μg PK-TRF0214-D 100 μg PK-TRF0214-M LANCE Eu-W1024 phospho-PKA sustrate antibody 10 μg PK-AD0182 LANCE Eu-W1024 phospho-(Ser) PKC substrate antibody 10 μg PK-AD0188 LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide Antibody 10 μg PK-TRF0207-D 100 μg PK-TRF0207-M LANCE Ultra Europium-anti-phospho-PLK (Ser137) antibody 10 μg PK-TRF0203-D 100 μg PK-TRF0203-M LANCE Eu-W1024 phospho-serine antibody (Zymed) 10 μg PK-AD0186 LANCE Eu-W1024 phospho-serine/threonine antibody 10 μg PK-AD0176 LANCE Eu-W1024 phospho-serine/threonine-phenylalanine antibody 10 μg PK-AD0178 LANCE Eu-W1024 phospho-serine/threonine-proline antibody 10 μg PK-AD0180 LANCE Eu-W1024 phospho-threonine antibody 10 μg PK-AD0094 1 mg PK-AD0095 LANCE Eu-W1024 phospho-threonine-proline antibody 10 μg PK-AD0099 1 mg PK-AD0100 LANCE Ultra Europium-anti-phospho-Tyrosine Hydroxylase (Ser40) antibody 10 μg PK-TRF0204-D 100 μg PK-TRF0204-M

ULight-labeled Peptide Substrate

Product Pack Size Cat. No. LANCE Ultra ULight-phospho-40S ribosomal protein S6 (pSer235/236) Peptide 0.5 nmole PK-TRF0129-D 5 nmole PK-TRF0129-M LANCE Ultra ULight-Acetyl-CoA Carboxylase (Ser79) Peptide 0.5 nmole PK-TRF0118-D 5 nmole PK-TRF0118-M LANCE Ultra ULight-AKT/PKB (Ser473) Peptide 0.5 nmole PK-TRF0124-D 5 nmole PK-TRF0124-M LANCE Ultra ULight-Cdc25C (Ser216) Peptide 0.5 nmole PK-TRF0123-D 5 nmole PK-TRF0123-M LANCE Ultra ULight-CREBtide 0.5 nmole PK-TRF0107-D 5 nmole PK-TRF0107-M LANCE Ultra ULight-DNA Topoisomerase 2-alpha (Thr1342) Peptide 0.5 nmole PK-TRF0130-D 5 nmole PK-TRF0130-M LANCE Ultra ULight-eIF4E-binding protein 1 (Thr37/46) Peptide 0.5 nmole PK-TRF0128-D 5 nmole PK-TRF0128-M LANCE Ultra ULight-Glycogen Synthase (Ser641/pSer657) Peptide 0.5 nmole PK-TRF0131D 5 nmole PK-TRF0131-M LANCE Ultra ULight-Crosstide (GSK-3α Ser21) 0.5 nmole PK-TRF0106-D 5 nmole PK-TRF0106-M LANCE Ultra ULight-Histone H3 (Thr3/Ser10) Peptide 0.5 nmole PK-TRF0125-D 5 nmole PK-TRF0125-M LANCE Ultra ULight-IkappaB-alpha (Ser32/36) peptide 0.5 nmole PK-TRF0113-D 5 nmole PK-TRF0113-M LANCE Ultra ULight-MBP peptide 0.5 nmole PK-TRF0109-D 5 nmole PK-TRF0109-M LANCE Ultra ULight-mTOR (Ser2448) Peptide 0.5 nmole PK-TRF0119-D 5 nmole PK-TRF0119-M LANCE Ultra ULight-p70 S6K (Thr389) Peptide 0.5 nmole PK-TRF0126-D 5 nmole PK-TRF0126-M LANCE Ultra ULight-PKC substrate 0.5 nmole PK-TRF0108-D 5 nmole PK-TRF0108-M LANCE Ultra ULight-PLK (Ser137) peptide 0.5 nmole PK-TRF0110-D 5 nmole PK-TRF0110-M LANC EUltra ULight-Tyrosine Hydroxylase (Ser40) peptide 0.5 nmole PK-TRF0111-D 5 nmole PK-TRF0111-M

LANCE Buffers

Product Pack Size Cat. No. LANCE Detection Buffer, 10X 250 ml PK-CR97-100 Stabilizer, 7.5% (DTPA-purified BSA) 50 ml PK-CR84-100

LANCE Ultra KinaSelect Kits - 可供測試找尋待測激酶的最佳 ULight-labeled Peptide Substrate

Product Pack Size Cat. No. LANCE Ultra KinaSelect Ser/Thr Kit 5 x 250 assay PK-TRF0300-C LANCE Ultra KinaSelect-TK Kit 1,000 assay PK-TRF0301-D