QCell-Eva One-Step qRT-PCR SuperMix Kit

• 針對 SYBR Green QPCR 偵測系統所設計的便利性產品，實驗者可不需事先經過 1st strand cDNA 合成步驟即可以單一步驟完成 RNA 或細胞 (不需事先萃取出 RNA) 樣本的 qRT-PCR 定量反應，偵測靈敏度可達 1 cell/ 1 pg total RNA。

• 有別於傳統 Syber Green 反應試劑，本產品中所含特殊 "Eva 螢光染劑"，與雙股 DNA 具有更高的親合性，因此可產生更強的螢光訊號提高實驗靈敏度。

• Eva 螢光染劑具有與 Syber Green 類似的激發/放射螢光波長，實驗者可以同樣的參數設定進行訊號偵測。

• 試劑隨附 Cell lysis buffer、Control human GAPDH primer set、ROX reference dye 讓實驗設計更加完整彈性。

QCell-Eva One-Step QRT-PCR SuperMix provides sensitive detection down to a single cell. K562 cells were lysed according to the cell lysis protocol. 4-fold serial dilution of cell lsyate were prepared from 64 cells to 1 cell. GAPDH gene expression was detected using QCell-Eva qRT-PCR kit on Stratagene's Mx3005P instrument. Efficiency as measured from standard curve was 102.3%, with a R2 value of 1.000.

訂購資訊