Western Blot Enhancing Kit
- 內含獨特 Western Blot 訊號放大試劑「Millenium Enhancer」，可增強抗原與抗體之間的親合性進而提高偵測靈敏度。
- 可搭配市面上任一款 ECL 化學冷光試劑使用。
Attoglow Western Blot Analysis Kit is an ultra-sensitive chemiluminescent Western blot assay. It detects protein at attogram (10-18 g) or yoctomole (10-24 M) levels. One ng of purified Rabbit muscle GAPDH was serially diluted to 1 attogram and electrophoresis was performed. The gel was transferred to a PVDF membrane, and then cut in the middle. The right section was treated with Millennium Enhancer, and the left was untreated. Immunoblotting was done using a primary antibody (clone 6C5) and HRP-conjugated rabbit anti-mouse antibody, and chemiluminescent substrates were applied. A film exposed for two minutes is shown above. 10-11 g of antigen without treatment and 10-14 g of GAPDH with treatment gave similar signal strengths, indicating a 1000-fold enhancement*.
*Fold enhancement varied from a few- to a 1000-fold depending on the characteristics of antibodies and antigen used.
*Fold enhancement varied from a few- to a 1000-fold depending on the characteristics of antibodies and antigen used.
訂購資訊
| Product Name | Pack Size | Cat. No. |
|---|---|---|
| Western Blot Enhancing Kit | for 1200 cm² membrane | BC-K3172120 |
| for 2500 cm² membrane | BC-K3172250 |
