qScript One-Step SYBR Green RT-qPCR

• 便利的 RT-qPCR 試劑套組，可於同一試管中完成 First-strand cDNA 合成與基因定量偵測。
  
• 僅需加入 RNA 樣本與基因專一性 Primers 即可進行反應。
  
• 採用 SYBR Green I 螢光染劑，提供高度靈敏的定量結果，線性偵測範圍廣泛 ( 1 pg - 100 ng total RNA )。
  
• 採用超高純度 Hot-start polymerase ，避免產生非專一性 PCR 產物。
  
• 提供混合有 ROX* 的產品選擇。 (※ ROX reference dye 可用於校正非 PCR 反應所導致的螢光訊號變化，標準化微量多孔盤 well 與 well 之間、以及不同批次實驗、不同偵測機型間的誤差)

產品效能：

One-Step SYBR Green RT-qPCR with Broad Dynamic Range, High Sensitivity and High Specificity. A 202 bp fragment of glyceraldehyde-3-phosphate dehydrogenase (GAPD) mRNA was amplified from log-fold serial dilutions of HeLa cell total RNA (100 ng to 0.1 pg). Eight replicate reactions for each RNA quantity, and the no template control (NTC) were carried out in 25 µl volumes with the qScript One-Step SYBR Green RT-qPCR Kit and 200 nM each GAPD specific primers (PrimerBank ID 7669492a2, Wang, X. and Seed, B (2003) NAR 31(24): e154; pp.1-8). Reactions were assembled on ice, transferred to a MyiQ™ real-time detection system (Bio-Rad Laboratories), and incubated for 5 min at 50°C followed by 2 min at 95°C. PCR cycling was for 40 cycles of 3 s, 95°C; 30 s, 60°C. Immediately following PCR cycling the block temperature was ramped from 60°C to 95°C and melt curve data was collected. Panel A) Amplification plots and standard curve regression analysis. Panel B) Dissociation results (melt curve) for NTC, 0.1 pg and 1 pg reactions.

訂購資訊