µ-Slide I Luer
- 多種微通道高度可選，可模擬不同血管壁壓力環境。
- 一體成型設計，省去自行組裝的麻煩，更可免除滲漏風險。
- 擁有優異的光學特性，可輕鬆取得高畫質細胞影像。
- 可彈性搭配針筒式、蠕動式、氣動式幫浦使用。
- 搭配 ibidi Pump 使用，可大幅減少細胞與培養液用量。
- 適合流體環境細胞培養 (cell culture under flow)、活細胞影像觀察攝影 (live cell imaging)、免疫螢光染色 (immunofluorescence) …等實驗使用。
歡迎與我們聯繫索取更多 µ-Slide I Luer 產品資訊與文獻。
產品特點
µ-Slide I Luer 具有多種微通道高度可選，可模擬不同血管壁壓力環境
ibidi Channel Slides 微流體玻片讓流體環境細胞培養實驗變得更簡單，不需繁複的組裝步驟，隨取隨用且無培養液滲漏風險
操作流程：(1) 打開玻片包裝，將細胞種入；(2) 待細胞貼附長滿後，於玻片兩端接上幫浦；(3) 於顯微鏡下觀察即時或是終端細胞影像。
從細胞培養、染色到顯微鏡觀察“All-in-one”完成，一氣呵成高效便利
The ibidi Channel Slides (e.g., µ-Slide VI 0.1 | µ-Slide VI 0.4 | µ-Slide VI 0.5 Glass Bottom | µ-Slide I Luer) are particularly suitable for immunofluorescence stainings. The geometry of the ibidi Channel Slides is ideal for the exact exchange of small medium amounts, which is necessary during immunocytochemistry stainings. In addition, the coverslip bottom of the channel μ-Slides eliminates the need for additional coverslips.
微通道結構可避免因表面張力產生弧形液面的現象，光線可筆直穿透，整個觀察視野亮度均勻，影像清晰不變形
Open well: The meniscus is disturbing the phase contrast effect. Only the center provides convenient contrast. ibidi Channel Slides: In a channel geometry the beam path is always aligned. Phase contrast microscopy is possible independent from the location.
底部厚度符合 #1.5 蓋玻片，影像清晰易觀測
Human umbilical vein endothelial cells (HUVEC) cultured under flow conditions (20 dyn/cm²) in a µ-Slide I 0.4 Luer over 9 days. The primary cells were transduced with the adenoviral vector rAV CMV-LifeAct-TagRFP 24 hours prior to the experiment.
產品規格
µ-Slide I Luer 規格比較
| µ-Slide I Luer | µ-Slide I Luer Glass Bottom | |
|---|---|---|
| Bottom | ibidi Polymer Coverslip | Glass Coverslip No. 1.5H |
| 0.2 Channel height | 200 μm | 250 μm |
| 0.2 Channel volume | 50 μl | 62.5 μl |
| 0.4 Channel height | 400 μm | 450 μm |
| 0.4 Channel volume | 100 μl | 112.5 μl |
| 0.6 Channel height | 600 μm | 650 μm |
| 0.6 Channel volume | 150 μl | 162.5 μl |
| 0.8 Channel height | 800 μm | 850 μm |
| 0.8 Channel volume | 200 μl | 212.5 μl |
| Outer dimensions | W25.5 x L75.5 mm | |
| Channel length | 50 mm | |
| Channel width | 5.0 mm | |
| Adapters | Female Luer | |
| Volume per reservoir | 60 µl | |
| Growth area | 2.5 cm² | |
| 0.2 Coating area | 5.2 cm² | |
| 0.4 Coating area | 5.4 cm² | |
| 0.6 Coating area | 5.6 cm² | |
| 0.8 Coating area | 5.8 cm² | |
底部材質規格比較
| #1.5 ibidi Polymer Coverslip (ibidi 特殊塑料) |
#1.5H ibidi Glass Coverslip (#1.5H 高精度玻璃) |
|
|---|---|---|
| Bottom thickness | 180 µm (+10/-5 µm) | 170 µm (+/-5 µm) |
| Bottom material | Polymer | D 263 M Schott high precision glass |
| Gas permeability | Yes | No |
| Compatibility with protein coatings | Yes | Yes |
| Immersion oil compatibility | Yes | Yes |
| Autofluorescence | Low | Low |
| Transmission | Very high (even ultraviolet) | High (ultraviolet restrictions) |
| Refractive index (nD 589 nm) | 1.52 | 1.52 |
| Brightfield Microscopy | ++ | ++ |
| Phase Contrast | ++ | ++ |
| Differential Interference Contrast (DIC) | ++ | ++ |
| Widefield Fluorescence | ++ | ++ |
| Confocal Fluorescence | ++ | ++ |
| Total Internal Reflection Fluorescence (TIRF) | + | ++ |
| Super-Resolution Microscopy | + | ++ |
※更多材質特性比較資訊請見這裡。
訂購資訊
| Product Name | Surface | Pack Size | Cat. No. |
|---|---|---|---|
| μ-Slide I 0.2 Luer | ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized | 15 | IB-80166 |
| Collagen IV: #1.5 polymer coverslip, sterilized | 15 | IB-80162 | |
| Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized | 15 | IB-80161 | |
| μ-Slide I 0.4 Luer | ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized | 15 | IB-80176 |
| Collagen IV: #1.5 polymer coverslip, sterilized | 15 | IB-80172 | |
| Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized | 15 | IB-80171 | |
| μ-Slide I 0.6 Luer | ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized | 15 | IB-80186 |
| Collagen IV: #1.5 polymer coverslip, sterilized | 15 | IB-80182 | |
| Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized | 15 | IB-80181 | |
| μ-Slide I 0.8 Luer | ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized | 15 | IB-80196 |
| Collagen IV: #1.5 polymer coverslip, sterilized | 15 | IB-80192 | |
| Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized | 15 | IB-80191 | |
| µ-Slide I Luer Variety Pack 四種微通道高度 (0.2/0.4/0.6/0.8 mm) 各 4 片 |
ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized | 16 | IB-81106 |
| Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized | 16 | IB-81101 | |
| μ-Slide I 0.2 Luer Glass Bottom | Glass Bottom: #1.5H (170 μm ±5 μm) D 263 M Schott glass, sterilized | 15 | IB-80167 |
| μ-Slide I 0.4 Luer Glass Bottom | Glass Bottom: #1.5H (170 μm ±5 μm) D 263 M Schott glass, sterilized | 15 | IB-80177 |
| μ-Slide I 0.6 Luer Glass Bottom | Glass Bottom: #1.5H (170 μm ±5 μm) D 263 M Schott glass, sterilized | 15 | IB-80187 |
| μ-Slide I 0.8 Luer Glass Bottom | Glass Bottom: #1.5H (170 μm ±5 μm) D 263 M Schott glass, sterilized | 15 | IB-80197 |
