µ-Slides With Test µ-Patterns

單一玻片含多種不同表面間隔圖案設計，以便測試最佳適用類型。
可使癌細胞株、球狀體細胞 (spheroids)、類器官 (organoids) …間隔排列生長於玻片上，進行 2D 或 3D 細胞培養與影像觀察。
具有 µ-Slide VI 0.4 與 µ-Slide 8 Well high 兩種產品款式可選。
µ-Slide VI 0.4 搭配 ibidi Pump 使用，可進行流體環境細胞培養 (cell culture under flow)。
由低自發螢光材質製成，且底部厚度符合 #1.5 蓋玻片標準，可直接結合高倍率光學或螢光顯微鏡進行細胞影像觀察。

本產品亦可提供客製化服務，歡迎與我們聯繫索取詳細資訊。

技術原理

ibidi µ-Patterning 技術原理是先將一般 µ-Slide VI 0.4 或 µ-Slide 8 Well high 產品底部以特殊材質「Polyol hydrogel」處理，使其形成完成不具附著性的 Bioinert 表面。接著以共價鍵結的方式將源自於纖連蛋白 (Fibronectin) 結合區域的 RGD 三胜肽 (Arg-Gly-Asp, RGD tripeptide) 固定在 Bioinert 表面，使其形成供細胞附著生長的區域；根據 RGD 鍵結區域的大小與排列，可形成各種不同圖案設計。以上圖為例，紅色 ibidi 字樣為表面鍵結有 RGD peptides 的 µ-Pattern 區域，黑色則為不具有附著性的 Bioinert 區域，可以觀察到細胞僅會在 µ-Pattern 區域附著生長。

產品特點

µ-Pattern 特寫

依據所選擇的產品款式 (Test Patterns 1, 2, 3)，單一玻片上會含有 6~12 種不同 µ-Patterns 圖案設計。上圖顯示的是各種 µ-Patterns 在玻片上的分佈位置，點擊這裡可瀏覽各 µ-Patterns 的詳細尺寸與設計。

底部厚度符合 #1.5 蓋玻片，影像清晰易觀測

Spheroid Generation. Suspension of NIH-3T3 cell line seeded on 200 µm adhesion spots, 64 hours live cell imaging, phase contrast, 4x objective lens.

Immunofluorescence staining of Human Umbilical Artery Endothelial Cells (HUAEC) cultivated in the µ-Slide VI 0.4 With Test µ-Pattern #10 (200 µm circles, 600 µm center-to-center distance, hexagonal layout). The cells were cultivated for two days before fixation and staining. Red: F-Actin cytoskeleton (phalloidin). White: nuclei (DAPI). Widefield fluorescence microscopy using a Zeiss Axio Observer.Z1 / 7, 10x objective lens. Courtesy of Jerome Jatzlau & Paul-Lennard Mendez, Knaus group, Institute of Chemistry and Biochemistry, FU Berlin, Germany.

Immunofluorescence staining of Human Aortic Endothelial Cells (HAoECs) cultivated in the µ-Slide VI 0.4 With Test µ-Pattern #11d (short lines, 200 µm x 10 µm, 90°). The cells were cultivated for two days before fixation and staining. Red: F-actin cytoskeleton (phalloidin). Green: adherens junctions (VE-cadherin). White: nuclei (DAPI). Widefield fluorescence microscopy using a Zeiss Axio Observer.Z1 / 7, 10x objective lens. Courtesy of Jerome Jatzlau & Paul-Lennard Mendez, Knaus group, Institute of Chemistry and Biochemistry, FU Berlin, Germany.

RCC-26 (human renal carcinoma) cells 24 hours after seeding in the µ-Slide VI 0.4 With Test µ-Pattern #14. Phase contrast microscopy, 4x objective lens.

Immunofluorescence staining of RCC-26 (human renal carcinoma) cells 24 hours after seeding in the µ-Slide VI 0.4 With Test µ-Pattern #14. Green: F-actin (phalloidin), red: α-Tubulin, blue: nuclei (DAPI). Widefield fluorescence microscopy, 10x objective lens.

Immunofluorescence staining of RCC-26 (human renal carcinoma) cells 24 hours after seeding in the µ-Slide VI 0.4 With Test µ-Pattern #15, Green: F-actin (phalloidin), red: α-Tubulin, blue: nuclei (DAPI). Widefield fluorescence microscopy, 60x objective lens.

產品規格

µ-Slide geometry	See product page µ-Slide VI 0.4 or µ-Slide 8 Well high
Binding motif	RGD
Pattern shape	25 variations
Surface passivation	Bioinert

訂購資訊

Product Name	µ-Pattern	Surface	Pack Size	Cat. No.
μ-Slide VI 0.4 μ-Pattern RGD Test Patterns 1	RGD, 15 different patterns, Test Patterns 1	#1.5 polymer coverslip, micropatterned surface, surface passivation with Bioinert, sterilized	2	IB-83651
μ-Slide VI 0.4 μ-Pattern RGD Test Patterns 2	RGD, 6 different patterns, Test Patterns 2	#1.5 polymer coverslip, micropatterned surface, surface passivation with Bioinert, sterilized	2	IB-83652
μ-Slide VI 0.4 μ-Pattern RGD Test Patterns 3	RGD, 6 different patterns, Test Patterns 3	#1.5 polymer coverslip, micropatterned surface, surface passivation with Bioinert, sterilized	2	IB-83653
μ-Slide 8 Well high μ-Pattern RGD Test Patterns 1	RGD, 15 different patterns, Test Patterns 1	#1.5 polymer coverslip, micropatterned surface, surface passivation with Bioinert, sterilized	2	IB-83851
μ-Slide 8 Well high μ-Pattern RGD Test Patterns 2	RGD, 6 different patterns, Test Patterns 2	#1.5 polymer coverslip, micropatterned surface, surface passivation with Bioinert, sterilized	2	IB-83852
μ-Slide 8 Well high μ-Pattern RGD Test Patterns 3	RGD, 6 different patterns, Test Patterns 3	#1.5 polymer coverslip, micropatterned surface, surface passivation with Bioinert, sterilized	2	IB-83853

哪些細胞適用 µ-Pattern RGD 產品？

基本上只要能在 Fibronectin 處理表面生長的細胞，都能適用於 µ-Pattern RGD 產品，目前測試過的細胞包含：A549 (human lung carcinoma), ARPE-19 (human retinal pigmented epithelium), BEAS-2B (human lung epithelium), CHO (Chinese hamster ovary), HEK-293 (human embryonic kidney), HeLa (human cervical carcinoma), HT-1080 (human fibrosarcoma), HuH-7 (human liver carcinoma), L929 (murine fibroblasts), MCF-10A (human breast epithelium), MDA-MB-231 (human breast carcinoma), MDA-MB-436 (human breast carcinoma), MDCK.2 (canine kidney cells), NCI-H441 (human lung adenocarcinoma), NIH-3T3 (murine fibroblasts), RCC-26 (human renal carcinoma)。建議可先購買 2 片小包裝產品，測試您細胞在 µ-Pattern RGD 產品的生長情形。