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 HRP 酵素冷光試劑
- 可與 HRP (horseradish peroxidase) 酵素作用反應後產生化學冷光。
- 多種產品規格可選，彈性符合各種實驗需求。
- Western Lightning
- Attoglow
Western Lightning series HRP chemiluminescent substrates
 由 Revvity 公司 (源自 PerkinElmer 生命科學和診斷部門) 所出品的 Western Lightning 系列 HRP 酵素冷光試劑，具有穩定優異的品質以及經濟合理的售價，是本公司長年暢銷不墜的明星商品之一。可區分為下列三類產品：
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Western Lightning Plus: 具有二倍於一般標準 ECL 冷光試劑的偵測靈敏度，可廣泛適用在大部分的蛋白質偵測實驗。品質穩定且價格實惠，適合日常 Western Blot 實驗使用！
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Western Lightning ECL ProNEW: 針對 CCD 影像擷取系統的使用者所設計，具有寬廣的可偵測範圍，無論是低含量或高含量蛋白質訊號，皆能清晰呈現！並具備有絕佳的訊號持久度，不論是長時間或多次曝光，皆皆能維持訊號的穩定性，大幅提昇使用者的實驗便利度與彈性。
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Western Lightning Ultra: 超高靈敏度 Western 冷光試劑，偵測靈敏度可達 Femtogram (10-15 g) 等級，可減少珍貴樣本或抗體使用量，訊號維持時間更長達 8 個小時。與市場其他同質商品相比，具有更加穩定的產品性能以及合理實惠的價格。
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 Lysate prepared from HEK293 cells using the AlphaScreen SureFire Lysis buffer. Lanes 2-9: 2-fold dilution series of lysate. Transfer performed using eBlot semi-dry transfer system. Blocked for 1 hour with blocking solution (PKI). Incubates ON at 4 oC with anti-AKT antibody at 1:1000 dilution (CST). Incubated 1 hour at RT with anti-rabbit0HRP at 1:100,000 dilution (PKI). 1 min exposure. |
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Western Lightning Plus allows you to detect targets that you may miss with standard products.
Western blots, serial dilutions of peanut lectin, rabbit anti-peanut lectin antibody, goat anti-rabbit HRP, 1-minute exposures. |
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Outstanding sensitivity with stable signal. The signal from Western Lightning Ultra remains stable over time, making it very tolerant of normal variability in workflow and ideal for repeated exposures.
*GAR HRP dilution
1-minute exposures taken at time intervals after substrate incubation. Slot blots, fivefold serial dilutions of rabbit IgG starting at 100 ng. Goat anti-rabbit HRP dilution following substrate manufacturer’s recommended conditions. |
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Good results with less optimization. Other high-sensitivity products can be “finicky,” requiring careful optimization. Western Lightning Ultra provides outstanding results even when conditions are not optimal.
Western blots, initial conditions: serial dilutions of C2C12 cell lysates, 10 μl sample, rabbit anti-total AKT 1:2,000, anti-rabbit HRP 1:100,000,
1-minute exposures. |
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訂購資訊
| Product |
Pack Size |
Cat. No. |
| Western Lightning Plus |
4 x 170 ml |
PK-NEL105 |
| Western Lightning ECL Pro |
4 x 170 ml |
PK-NEL122 |
| Western Lightning Ultra |
2 x 10 ml |
PK-NEL111 |
| 2 x 55 ml |
PK-NEL112 |
| 2 x 110 ml |
PK-NEL113 |
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Attoglow series HRP chemiluminescent substrates
由 BioChain 公司所出品的 Attoglow 系列 HRP 酵素冷光試劑，偵測靈敏度可達 Attogram (10-18 g) 等級。內含獨家 Millenium Enhancer 試劑，可增強抗原與抗體之間的親合性，進而提高偵測靈敏度。可區分為下列二類產品：
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Attoglow Western Blot Analysis Kit: 內含 Millenium Enhancer、冷光試劑、二級抗體、阻斷液 (blocking solution) 等完整試劑組件，使用更便利。
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AttoglowAdvance Western Blot Analysis Kit: 偵測靈敏度優於 Attoglow Western Blot Analysis Kit，可用來偵測極微量的蛋白質。
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訂購資訊
| Product |
Pack Size |
Cat. No. |
| Attoglow Western Blot Analysis Kit |
| Attoglow Western Blot Analysis Kit with Anti mouse secondary antibody |
for 1200 cm2 membrane |
BC-K3171120-I |
| for 2500 cm2 membrane |
BC-K3171250-I |
| Attoglow Western Blot Analysis Kit with Anti rabbit secondary antibody |
for 1200 cm2 membrane |
BC-K3171120-II |
| for 2500 cm2 membrane |
BC-K3171250-II |
| Attoglow Western Blot Analysis Kit with Anti chicken secondary antibody |
for 1200 cm2 membrane |
BC-K3171120-III |
| for 2500 cm2 membrane |
BC-K3171250-III |
| Attoglow Western Blot Analysis Kit with Anti goat secondary antibody |
for 1200 cm2 membrane |
BC-K3171120-IV |
| for 2500 cm2 membrane |
BC-K3171250-IV |
| AttoglowAdvance Western Blot Analysis Kit |
| AttoglowAdvance Western Blot Analysis Kit with Anti mouse secondary antibody |
for 1200 cm2 membrane |
BC-K3173120-I |
| AttoglowAdvance Western Blot Analysis Kit with Anti rabbit secondary antibody |
for 1200 cm2 membrane |
BC-K3173120-II |
| AttoglowAdvance Western Blot Analysis Kit with Anti chicken secondary antibody |
for 1200 cm2 membrane |
BC-K3173120-III |
| AttoglowAdvance Western Blot Analysis Kit with Anti goat secondary antibody |
for 1200 cm2 membrane |
BC-K3173120-IV |
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