µ-Slide ibiPore SiN
µ-Slide ibiPore SiN 具備兩條可培養細胞的通道，通道交疊處採多孔陶瓷膜設計，能將傳統使用 Transwell (Boyden chamber) 進行的各式 Transmigration & Transport 試驗轉移至流體環境下進行，模擬循環系統，使測試情境更貼近生理環境！其產品特性包含：
- 搭配幫浦（例如 ibidi Pump System）不同剪切應力設定，可模擬生理上不同流體環境，如動脈、靜脈、肺泡等。
- 陶瓷膜具極佳光學性質，可輕易獲得清晰顯微鏡成像。
- 針對不同細胞、不同應用，提供多種陶瓷膜孔徑大小可選。
- 符合標準載玻片規格（W25.5 x L75.5 mm），廣泛適用各類倒立式顯微鏡。
產品用途
- 通過多孔膜的細胞遷移 (Transmigration)、趨化 (Chemotaxis)、侵入 (Invasion) 試驗
- 流體環境 3D 細胞培養
- 細胞極化試驗 (apical-basal cell polarity assays)
- 肺泡細胞或皮膚細胞微環境模擬
- 血腦障壁 (blood brain barrier) 模擬
歡迎與我們聯繫索取更多 µ-Slide ibiPore SiN 產品資訊與文獻。
產品特點
µ-Slide ibiPore SiN 可搭配 ibidi Pump 使用，於流體環境下進行各式 Transmigration & Transport 試驗
The µ-Slide ibiPore SiN allows you to perform several cell culture assays with a porous membrane. (A) Endothelial Barrier Assays. Cells on the lower side of the membrane with defined shear stress. (B) Cell Barrier and Co-Culture Assays. Co-culture of two cell types on both sides of the membrane. (C) Cell Transport in a 3D Gel Matrix. Transmigration of cells across a cell monolayer on the membrane.
可依據研究用途，選擇不同陶瓷膜孔徑大小進行實驗
| 應用實驗 | 細胞類型 | 推薦孔徑 |
|---|---|---|
| Permeability and transport studies | Endothelial, epithelial | 0.5 µm | 3 µm |
| Cell polarity assays | Endothelial, epithelial, kidney | 0.5 µm | 3 µm |
| Co-culture assays | Fibroblasts, endothelial, epithelial, cancer, stem cells | 0.5 µm | 3 µm |
| Transmigration, chemotaxis assays | Endothelial, leukocytes (neutrophils), lymphocytes (T cells, B cells) | 3 µm | 5 µm | 8 µm |
| Invasion, migration, and metastatic potential assays | Monocytes, macrophages, lymphocytes (T cells, B cells), cancer, endothelial, epithelial, fibroblasts, osteoblasts | 3 µm | 5 µm | 8 µm |
µ-Slide ibiPore SiN 底部厚度皆符合 #1.5 蓋玻片，影像清晰易觀測
(A) Immunofluorescence staining of human endothelial cells in the µ-Slide ibiPore, 3 µm pore size, with collagen IV coating. Overlay image of phase contrast, DAPI (blue), VE-cadherin (green), and F actin (red). (B) Immunofluorescence staining of human umbilical vein epithelial cells (HUVEC) in the µ-Slide ibiPore SiN, 5 µm pore size, with fibronectin coating. Cells were seeded and kept for 12 h in the incubator under flow conditions (10 dyne/cm²) with the ibidi Pump System. Green: F actin (phalloidin), blue: nuclei (DAPI). Fluorescence microscopy, 20x objective. (C) Human umbilical vein epithelial cells (HUVEC) in the µ-Slide ibiPore SiN, 3 µm pore size, with fibronectin coating. Cells were seeded and kept for 12 h in the incubator under flow conditions (10 dyne/cm²) with the ibidi Pump System. Phase contrast microscopy after fixation, 10x objective.
產品規格
| Geometry of the µ-Slide ibiPore SiN | |
| Total coating area | 4.50 cm² |
| Bottom | ibidi Polymer Coverslip |
| Main Channel (Lower Channel) | |
| Access | Luer port, accessible with female Luers |
| Volume | 50 µl |
| Dimensions | W5 x L25 x H0.4 mm |
| Growth area | 1.25 cm² |
| Upper Channel | |
| Access | Reservoir port, accessible with 20/200 µl pipet tipsm |
| Volume | 55 µl |
| Height over membrane | 1.3 mm |
| Growth area | 0.81 cm² |
| ibiPore Membrane | |
| Material | Silicon nitride (SiN) |
| Thickness | 0.4 µm |
| Membrane size | 2 mm x 2 mm |
| Porous area | 1.77 mm x 1.84 mm |
| Restrictions for objective lenses | Working distance >0.5 mm |
| Pore layout | Hexagonal spacing |
| Available Variations |
|
訂購資訊
| Product Name | Pore Size | Porocity | Pack Size | Cat. No. |
|---|---|---|---|---|
| µ-Slide ibiPore SiN 0.5 µm/20% ibiTreat Trial Pack | 0.5 µm | 20%; | 2 | IB-85216-S |
| µ-Slide ibiPore SiN 0.5 µm/20% ibiTreat | 10 | IB-85216 | ||
| µ-Slide ibiPore SiN 3 µm/5% ibiTreat Trial Pack | 3 µm | 5% | 2 | IB-85226-S |
| µ-Slide ibiPore SiN 3 µm/5% ibiTreat | 10 | IB-85226 | ||
| µ-Slide ibiPore SiN 5 µm/5% ibiTreat Trial Pack | 5 µm | 5% | 2 | IB-85236-S |
| µ-Slide ibiPore SiN 5 µm/5% ibiTreat | 10 | IB-85236 | ||
| µ-Slide ibiPore SiN 8 µm/5% ibiTreat Trial Pack | 8 µm | 5% | 2 | IB-85246-S |
| µ-Slide ibiPore SiN 8 µm/5% ibiTreat | 10 | IB-85246 |
µ-Slide ibiPore SiN 和 µ-Slide I Luer 3D 產品比較表
雖然這兩種產品都能應用在流體環境 3D 細胞培養、細胞極化試驗、肺泡細胞或皮膚細胞微環境模擬、血腦障壁 (blood brain barrier) 模擬…等實驗，但由於產品結構設計上的不同，在使用上仍會有些區別與限制。歡迎洽詢伯森業務專員索取更多應用資訊與選擇指南。
 µ-Slide ibiPore SiN |
 µ-Slide I Luer 3D |
|
|---|---|---|
| Geometry |  |
 |
| Principle of interface | Membrane-based | Gel-based |
| Substrate for cells | Hard membrane with different pore sizes | Soft hydrogels (e.g., collagen or Matrigel) |
| Microscopy | No restriction | Restricted by gel’s optics |
| Flow application | Defined shear stress in the lower channel | Defined shear stress in the channel |
